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A2230

Apyrase from potatoes

High Activity, ATPase ≥600 units/mg protein, lyophilized powder

Synonim(y):

Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase

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Informacje o tej pozycji

Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
Numer WE:
MDL number:
Biological source:
potato


biological source

potato

form

lyophilized powder

quality

High Activity

ATPase activity

≥600 units/mg protein

secondary activity

≥50 % of base activity ADPase

composition

protein, ≥30%

foreign activity

Acid Phosphatase ≤2% of base activity

shipped in

wet ice

storage temp.

−20°C

Application

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation [1]. Product A2230 is a high activity apyrase.
At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+Pi → AMP+2Pi.

Biochem/physiol Actions

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis [2]. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

Packaging

Sold on the basis of ATPase units.

Physical form

Lyophilized powder containing potassium succinate buffer salts.

Preparation Note

Derived from red potato

Other Notes

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.
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Ta pozycja
A6132A6237SRE0019
biological source

potato

biological source

potato

biological source

-

biological source

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

wet ice

shipped in

-

shipped in

wet ice

shipped in

-

foreign activity

Acid Phosphatase ≤2% of base activity

foreign activity

-

foreign activity

-

foreign activity

Acid Phosphatase ≤2% of base activity

composition

protein, ≥30%

composition

Protein, 30-60%

composition

-

composition

Protein, 25-60%


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pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Klasa składowania

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Numer pozycji handlu globalnego

SKUNUMER GTIN
A2230-100UN04061833349861

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