SRE0019

Apyrase from potatoes

Suitable for manufacturing of diagnostic kits and reagents, ATPase ≥200 units/mg protein

• Synonim(y): Apyrase from potatoes, Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase
• Numer CAS: 9000-95-7
• Numer EC enzymu: 3.6.1.5 (BRENDA, IUBMB)
• Numer WE: 232-569-8
• Numer MDL: MFCD00130542
• Kod UNSPSC: 12352204
• NACRES: NA.32

Właściwości

• Poziom jakości: 400
• typ: Grade VII
• Postać: lyophilized powder
• ATPase activity: ≥200 units/mg protein
• secondary activity: ≥50 % of base activity ADPase
• skład: Protein, 25-60%
• rozpuszczalność: H₂O: soluble 1.0 mg/mL
• Zastosowanie: diagnostic assay manufacturing
• obecność zanieczyszczeń: Acid Phosphatase ≤2% of base activity
• temp. przechowywania: −20°C

Opis

Zastosowanie

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation.

At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+P_i → AMP+2P_i.

Działania biochem./fizjol.

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

Definicja jednostki

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

Postać fizyczna

Partially purified, lyophilized powder containing potassium succinate buffer salts.

Uwaga dotycząca przygotowania

Sold on the basis of ATPase units.

Inne uwagi

This grade has a low ATPase/ADPase ratio.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H334
• Zwroty wskazujące środki ostrożności: P261 - P284 - P501
• Klasyfikacja zagrożeń: Resp. Sens. 1
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable