MABE455

Anti-CHD-4 Antibody, clone 3F2/4

clone 3F2/4, from mouse

• Synonim(y): Chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 3F2/4, monoclonal
• reaktywność gatunkowa: mouse, human
• metody: ChIP: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• izotyp: IgG2aκ
• numer dostępu NCBI: NP_001264
• numer dostępu UniProt: Q14839
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... CHD4(1108)

Opis

Opis ogólny

CHD-4, often referred to as Mi2-beta, is a central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. CHD-4 has been shown to interact with TRIM27, HDAC1, Histone deacetylase 2, MTA2, SATB1 and Ataxia telangiectasia and Rad3 related. This protein also interacts with KLF1. Sumoylation of KLF1 leads to transcriptional repression of CHD-4.

Immunogen

Linear peptide corresponding to human CHD-4.

Zastosowanie

Detect Chd4 (Chromodomain-helicase-DNA-binding protein 4) using this mouse monoclonal antibody, Anti-CHD-4 Antibody, clone 3F2/4 validated for use in western blotting, IHC & Immunofluorescence.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected CHD-4 in human breast carcinoma and skin tissues.

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory detected CHD-4 in mouse embryonic endothelial cells.

Immunofluorescence Analysis: A representative lot from an independent laboratory detected CHD-4 in mouse embryonic endothelial cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Jakość

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected CHD-4 in 10 µg of HeLa cell lysate.

Opis wartości docelowych

~220 kDa observed

Postać fizyczna

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
HeLa cell lysate

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

This page may contain text that has been machine translated.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable