MABE920

Anti-Mi-2β Antibody, clone 17H11

clone 17H11, from mouse, purified by affinity chromatography

• Synonym(s): Chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, Mi-2b
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 17H11, monoclonal
• Application: ChIP, WB
• Citations: -

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 17H11, monoclonal
• purified by: affinity chromatography
• species reactivity: human
• technique(s): ChIP: suitable (ChIP-seq), western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_001264
• UniProt accession no.: Q14839
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CHD4(1108)

Description

General description

CHD-4, often referred to as Mi2-beta, is a central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. CHD-4 has been shown to interact with TRIM27, HDAC1, Histone deacetylase 2, MTA2, SATB1 and Ataxia telangiectasia and Rad3 related. This protein also interacts with KLF1. Sumoylation of KLF1 leads to transcriptional repression of CHD-4.

~ 220 kDa observed

Immunogen

Recombinant protein corresponding to Human Mi-2β (CHD-4).

Application

Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody as part of a monoclonal antibody cocktail was used for ChIP. See Zhang et al., 2012 for details.

ChIP-seq Analysis: A representative lot of this antibody as part of a monoclonal antibody cocktail was used for ChIP-seq. See Zhang et al., 2012 for details.

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Histone Modifying Proteins

This Anti-Mi-2β Antibody, clone 17H11 is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of Mi-2β.

Biochem/physiol Actions

Recognizes Mi-2β (CHD-4)

Physical form

Protein G Purified

Purified mouse monoclonal IgG2bκ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in a Jurkat cell lystate.

Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Mi-2β (CHD-4) in a Jurkat cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable