8.51025

5-Carboxyfluorescein

Novabiochem^®

• Synonim(y): 5-Carboxyfluorescein, 5-FAM
• Wzór empiryczny (zapis Hilla): C₂₁H₁₂O₇
• Numer CAS: 76823-03-5
• Masa cząsteczkowa: 376.32
• Numer MDL: MFCD00036874
• Kod UNSPSC: 12352005
• NACRES: NA.22

Właściwości

• Poziom jakości: 200
• linia produktu: Novabiochem^®
• Próba: ≥98% (TLC); ≥99.0% (HPLC)
• Postać: powder
• przydatność reakcji: reagent type: fluorescent-labelling reagent
• producent / nazwa handlowa: Novabiochem^®
• Zastosowanie: peptide synthesis
• temp. przechowywania: 2-8°C
• InChI: 1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)
• Klucz InChI: NJYVEMPWNAYQQN-UHFFFAOYSA-N

Opis

Opis ogólny

This high purity fluorescent dye (λ_ex494 nm, λ_em 518 nm) is supplied as a single isomer, which ensures production of labeled products of defined chemical structure, as well as greatly simplifying purification and characterization. FAM should be introduced as the last step in the synthesis to avoid formation of side-products, or be protected by tritylation prior to acylation or hydrazine treatment ^[1].

Associated Protocols and Technical Articles
Chromogenic Labeling of Synthetic Peptides

Literature references

^[1] R. Fischer, et al. (2003) Bioconjugate Chem., 14, 653.

Zastosowanie

5-Carboxyfluorescein Novabiochem^®. CAS 76823-03-5, molar mass 376.32 g/mol.

Powiązanie

Replaces: 01-63-0112

Komentarz do analizy

Colour (visual): yellow to orange
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC (EtOAc/MeOH/H2O/DIPEA 60 : 30 : 10 : 3)): ≥ 98 %
Assay (HPLC, area%): ≥ 99.0 % (a/a)

To see the solvent systems used for TLC of Novabiochem^® products please click here.

Informacje prawne

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable