341220

Exo1

A cell-permeable methylanthranilate analog that reversibly inhibits vesicular traffic from ER to Golgi in mammalian cells by inducing tubulation and collapsing of the Golgi membrane.

• Synonim(y): Exo1, 2-(4-Fluorobenzoylamino)methylbenzoate, 2-(4-Fluorobenzoylamino)benzoic Acid Methyl Ester, ER Export Inhibitor I
• Wzór empiryczny (zapis Hilla): C₁₅H₁₂FNO₃
• Numer CAS: 75541-83-2
• Masa cząsteczkowa: 273.26
• UNSPSC Code: 12352200
• NACRES: NA.77
• MDL number: MFCD00028104
• Assay: ≥95% (HPLC)
• Form: solid
• Quality level: 100
• Storage condition: OK to freeze, protect from light

Właściwości

• Quality Level: 100
• assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, protect from light
• color: white
• solubility: DMF: 25 mg/mL, DMSO: 25 mg/mL, ethanol: 5 mg/mL, methanol: 5 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: Fc1ccc(cc1)C(=O)Nc2c(cccc2)C(=O)OC
• InChI: 1S/C15H12FNO3/c1-20-15(19)12-4-2-3-5-13(12)17-14(18)10-6-8-11(16)9-7-10/h2-9H,1H3,(H,17,18)
• InChI key: KIAPWMKFHIKQOZ-UHFFFAOYSA-N

Opis

General description

A cell-permeable methylanthranilate analog that reversibly inhibits vesicular traffic from ER to Golgi in mammalian cells (IC₅₀ = 20 µM in BSC1 cells) by inducing tubulation and collapsing of the Golgi membrane and redirecting the traffic back to ER. Acts as a potent and selective modifier of Golgi ARF1 GTPase activity. Unlike another membrane traffic inhibitor Brefeldin A (BFA; Cat. No. 203729), its effect seems to be limited to Golgi, as no effect can be detected on endocytic organelles, such as endosomes and TGN (trans-Golgi network). Its inhibitory mechanism and target(s) appear to be quite different from those of BFA and thus serves as a valuable alternative that complements BFA in membrane traffic studies.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Golgi ARF1 GTPase

Product does not compete with ATP.

Reversible: yes

Target IC₅₀: 20 µM inhibiting vesicular traffic from ER to Golgi in BSC1 cells

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Feng. Y., et al. 2003. Proc. Natl. Acad. Sci. USA100, 6469.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable