16-638
4G10® Platinum, Anti-Phosphotyrosine Antibody, Agarose Conjugate
clone 4G10®, Upstate®, from mouse
Synonim(y):
4G10 Platinum Anti-Phosphotyrosine, 4G10 Platinum Antibody, Phosphotyrosine Detection Antibody
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
rodzaj przeciwciała
primary antibodies
klon
4G10®, monoclonal
reaktywność gatunkowa (przewidywana na podstawie homologii)
all
producent / nazwa handlowa
Upstate®
metody
immunoprecipitation (IP): suitable
izotyp
IgG2bκ
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
phosphorylation (pTyr)
informacje o genach
human ... PID1(55022)
Opis ogólny
The development of the anti-phosphotyrosine, clone 4G10 in 1989 was a monumental discovery for researchers. 4G10 was the first and best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10 is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. Now Millipore has made the best even better with 4G10 Platinum. We pooled 4G10 with the next most highly regarded anti-phosphotyrosine, clone PY20, to make 4G10 Platinum. PY20 itself is a very poor substitute for 4G10, but its additive effect allow for a greater level of detection on more substrates that even 4G10 alone is capable.
Immunogen
The immunogen for 4G10 was phospho-tyramine coupled to KLH, while the immunogen for PY20 was phosphotyrosine conjugated to a carrier protein.
Zastosowanie
4G10 Platinum, Anti-Phosphotyrosine, Agarose Conjugate detects levels of Phosphotyrosine proteins & has been published & validated for use in IP.
Cechy i korzyści
Format: Gel Immobilized
Jakość
Immunoprecipitation:
Twenty μl of 50% slurry of this reagent (10 μl packed beads) was used to immunoprecipitate tyrosine-phosphorylated proteins from 250 μg PDGF-stimulated NIH3T3 cell lysate or EGF-stimulated A431 cell lysate.
Twenty μl of 50% slurry of this reagent (10 μl packed beads) was used to immunoprecipitate tyrosine-phosphorylated proteins from 250 μg PDGF-stimulated NIH3T3 cell lysate or EGF-stimulated A431 cell lysate.
Opis wartości docelowych
Dependent upon the molecular weight of the Tyrosine-phosphorylated protein
Postać fizyczna
Supplied as a 50% slurry of a proprietary mixture of agarose conjugated, protein G purified mouse monoclonal 4G10 (IgG2bκ) and protein A purified PY20 (IgG2b) in PBS, pH 7.4 with 0.05% sodium azide.
protein G purified mouse monoclonal 4G10 (IgG2bκ) and protein A purified PY20 (IgG2b)
Przechowywanie i stabilność
Stable for 6 months at 2-8°C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap.
NOTE: DO NOT FREEZE.
NOTE: DO NOT FREEZE.
Informacje prawne
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Certyfikaty analizy (CoA)
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