07-353
Anti-acetyl-Histone H3 (Lys14) Antibody
serum, Upstate®
Synonim(y):
H3K14Ac, Histone H3 (acetyl K14), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
serum
rodzaj przeciwciała
primary antibodies
klon
polyclonal
reaktywność gatunkowa
Saccharomyces cerevisiae, yeast, human
producent / nazwa handlowa
Upstate®
metody
ChIP: suitable (ChIP-seq)
dot blot: suitable
multiplexing: suitable
western blot: suitable
izotyp
IgG
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
acetylation (Lys14)
informacje o genach
human ... H3C1(8350)
Opis ogólny
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Acetylation of lysine14 is commonly seen in genes that are being actively transcribed into RNA.
Specyficzność
Broad species-cross reactivity is expected.
Recognizes acetyl-histone H3 (Lys14), Mr ~17 kDa. An additional unknown protein was detected at Mr ~33 kDa.
Immunogen
Epitope: a.a. 9-18
Ovalbumin-conjugated, synthetic peptide (KSTGGAcKAPRK-C) corresponding to amino acids 9-18 of yeast histone H3 acetylated on lysine 14, with a C-terminal cysteine added for conjugation purposes.
Zastosowanie
Anti-acetyl-Histone H3 (Lys14) Antibody is a rabbit polyclonal antibody for detection of acetyl-Histone H3 (Lys14) also known as H3K14Ac, Histone H3 (acetyl K14) & has been validated in ChIP, WB, ChIP-seq, DB, Mplex.
Chromatin Immunoprecipitation (ChIP):
An independent laboratory has shown this antibody preferentially immunoprecipitates chromatin from wild type yeast and not from yeast strains containing a Lysine substitution to Alanine at residue 14.
Beadlyte Histone-Peptide Specificity Assay:
1:1000-1:5000 dilutions of a previous lot were incubated with histone H3 peptides containing various modifications conjugated to Luminex microspheres. No cross-reactivity with peptides containing acetyl-lysine 9 or acetyl-lysine 27 was detected.
An independent laboratory has shown this antibody preferentially immunoprecipitates chromatin from wild type yeast and not from yeast strains containing a Lysine substitution to Alanine at residue 14.
Beadlyte Histone-Peptide Specificity Assay:
1:1000-1:5000 dilutions of a previous lot were incubated with histone H3 peptides containing various modifications conjugated to Luminex microspheres. No cross-reactivity with peptides containing acetyl-lysine 9 or acetyl-lysine 27 was detected.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Jakość
In acid extracts from sodium butyrate treated HeLa cells.
Western Blot Analysis:
A 1:1000-1:5000 dilution of this lot detected acetyl-Histone H3 (Lys14) in acid extracts from sodium butyrate treated HeLa cells (Catalog # 17-305).
Western Blot Analysis:
A 1:1000-1:5000 dilution of this lot detected acetyl-Histone H3 (Lys14) in acid extracts from sodium butyrate treated HeLa cells (Catalog # 17-305).
Opis wartości docelowych
17 kDa
Powiązanie
Replaces: 04-1044
Postać fizyczna
Antiserum containing 0.05% sodium azide and 30% glycerol.
Unpurified
Przechowywanie i stabilność
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Komentarz do analizy
Control
C6 cell lysate, NIH 3T3 cell lysate, human breast carcinoma and human lung carcinoma.
C6 cell lysate, NIH 3T3 cell lysate, human breast carcinoma and human lung carcinoma.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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