Przejdź do zawartości
Merck

Przejdź do

XNAPG

SYBR® Green Extract-N-Amp Plant PCR Kit

sufficient for 100 preparations

Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.

Wybierz wielkość

Zmień widok
Do Państwa/SKUDostępnośćCena netto
1 kit
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
1420,00 zł

Informacje o tej pozycji

NACRES:
NA.55
UNSPSC Code:
41106303

1420,00 zł


Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności

Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomóc


Quality Segment

usage

sufficient for 100 preparations, sufficient for 100 reactions

feature

dNTPs included, hotstart

technique(s)

RT-qPCR: suitable, qPCR: suitable

input

crude DNA

detection method

SYBR® Green

shipped in

wet ice

storage temp.

−20°C

General description

SYBR® Green Extract-N-Amp Plant PCR Kit is recommended for fast and reliable direct PCR from a wide range of plant leaf tissue.
The SYBR Green Extract-N-Amp Plant PCR Kit contains all the reagents needed for rapid extraction of genomic DNA from plant leaf tissue coupled with direct polymerase chain reaction (PCR) amplification and real-time detection. The need for freezing plant tissue in liquid nitrogen or mechanical disruption and complete genomic DNA purification has been eliminated.
Two solutions provided in the kit combine to release genomic DNA from leaf tissue and neutralize substances inhibitory to PCR. The genomic DNA released into solution serves as a template for the SYBR Green Extract-N-Amp PCR ReadyMix. This ReadyMix, which contains SYBR Green fluorescent dye, Taq, JumpStart antibody, dNTPs and MgCl2, is specially formulated to amplify target sequences in the cellular lysate.

Features and Benefits

  • Single-step extraction of plant genomic DNA for polymerase chain reaction (PCR) in less than 15 minutes
  • No freezing, mechanical disruption, organic extraction, column purification, or precipitation required
  • Specially formulated PCR ReadyMix for use with extract
  • Hot Start antibody for highly specific PCR amplification of genomic DNA
  • No loading buffers or tracking dyes required for gel analysis
  • SYBR® Green I dye acts as a nonspecific reporter for real-time PCR
  • SYBR® Green I dye is inexpensive, easy to use, and highly sensitive
  • Compatible with high-throughput requirements for genetic analysis of plants
  • No long enzymatic digestions
  • Extract stable at 4°C for at least 6 months

Other Notes

The protocol uses a 20 μl PCR reaction.

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries
Ta strona może zawierać tekst przetłumaczony maszynowo.

Porównaj podobne pozycje

Wyświetl pełne porównanie

Pokaż różnice

1 of 1

Ta pozycja
XNAP2XNATGXNATRG
technique(s)

RT-qPCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

detection method

SYBR® Green

detection method

-

detection method

-

detection method

-

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart, Direct PCR

feature

dNTPs included, hotstart, Direct PCR

feature

dNTPs included, hotstart, Direct PCR

usage

sufficient for 100 preparations, sufficient for 100 reactions

usage

sufficient for 100 amplifications, sufficient for 100 extractions, sufficient for 100 reactions

usage

sufficient for 100 amplifications, sufficient for 100 extractions, sufficient for 100 reactions

usage

sufficient for 1000 amplifications, sufficient for 1000 extractions, sufficient for 1000 reactions

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200


Elementy zestawu są też dostępne oddzielnie

Numer produktu
Opis
Karta charakterystyki & Cennik

  • Extraction Solution
    Karta charakterystyki

  • Dilution Solution
    Karta charakterystyki

  • SYBR® Green Extract-N-Amp PCR ReadyMix
    Karta charakterystyki

pictograms

Exclamation markEnvironment

signalword

Warning

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Klasa składowania

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3



Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Dokumenty section.

Proszę o kontakt, jeśli potrzebna jest pomoc Obsługa Klienta

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów



Questions

Reviews

No rating value

Active Filters