P6278

Peroxidase Inactivated

≤0.02 units/mg protein (biuret)

• Synonim(y): Peroxidase, inactivated from horseradish peroxidase
• UNSPSC Code: 12352204
• NACRES: NA.54
• MDL number: MFCD00071339
• Specific activity: ≤0.02 units/mg protein (biuret)

Właściwości

• form: powder
• Quality Level: 200, 300
• specific activity: ≤0.02 units/mg protein (biuret)
• impurities: salt, free
• solubility: 0.85% sodium chloride: soluble 15 mg/mL, clear to slightly hazy, colorless to light yellow
• storage temp.: 2-8°C

Opis

Application

Inactivated peroxidase is used to study regeneration of inactivated peroxidase enzymes. It has been used to study the mechanism of peroxidase solubilization[1] and it is used to study antitumor activity of immobilized peroxidase systems. It may be used for developing differential staining techniques in nerve tissue preparations.

Biochem/physiol Actions

Inactivated peroxidase reacts with goat and rabbit anti-peroxidase. It may be reactivated various methods such as heating.

HRP readily combines with hydrogen peroxide (H₂O₂) and the resultant [HRP-H₂O₂] complex can oxidize a wide variety of hydrogen donors. The optimal pH is 6.0-6.5 and the enzyme is most stable in the pH range of 5.0-9.0. HRP can be conjugated to antibodies by several different methods that include the use of glutaraldehyde, periodate oxidation, disulfide bonds, and also via amino- and thiol-directed cross-linkers. It is smaller and more stable than the enzyme labels, β-galactosidase and alkaline phosphatase. Hence, it is the most desired label. Also, its glycosylation leads to lower non-specific binding. It is also used for the determination of glucose and peroxides in solution. Sodium azide, cyanide, L-cystine, dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and ions such as Cd²⁺, Co²⁺, Cu²⁺, Fe³⁺, Mn²⁺, Ni²⁺, and Pb²⁺ are found to inhibit the enzyme activity.

Preparation Note

Prepared from Type VI horseradish peroxidase by a modification of the method of Teale.

Other Notes

One unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20°C.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)