Following reconstitution with 250 mL of water, the prepared 1X solution may be stored at room temperature for 3 months. Please see the link below to review additional information provided in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/355/046/g4410bul.pdf
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G4410
Griess reagent (modified)
Synonim(y):
Greiss, nitrite assay
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| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
10 g | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1270,00 zł |
Informacje o tej pozycji
UNSPSC Code:
12352200
EC Number:
215-981-2
NACRES:
NA.32
1270,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócGeneral description
Griess reagent is based on Griess reaction. The components include naphthylethylenediamine dihydrochloride suspended in water and sulphanilamide in phosphoric acid. This reagent reacts with nitrite in samples to form a purple azo product, absorbance of which is measured at 546 nm.
Application
Griess reagent (modified) has been used:
- in the nitrite assay of primary spinal cord glial cells stimulated with supernatant of damaged sensory neuron (SDSN)
- for measuring nitrite/nitrate oxidized from nitric oxide in stimulated chondrocytes
- for measuring nitric oxide (NO) degradation in RAW264.7 macrophage cells
- to measure the concentration of nitric oxide(NO) in the conditioned media from cultured macrophages (MΦs)
- to determine the relative levels of NO released by BEAS-2B bronchial cells in the culture supernatants
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|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level - | Quality Level 100 |
| form powder | form liquid | form - | form - |
| usage 10 g | usage - | usage sufficient for 200 colorimetric tests | usage - |
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1
Klasa składowania
8A - Combustible corrosive hazardous materials
wgk
WGK 2
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How to store prepared Griess Reagent that j dissolved in liquid? And how long its shelf life?
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1. Can the griess reagent solution be stored for longer if I keep it in the freezer? 2. Does my sample (a supernatant of cell culture media) has to be centrifuged first before I do the assay? 3. Do I add the reagent to my standard (NaNO2) as well?
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We have no information on storing this product in the freezer. Yes, it is best to spin your cell culture sample down before the assay. Yes, you will need to add the reagent to your standards.
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if we dissolve Griess reagent 40 mg/ml in water then what will be its concentration (molarity).
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This product is a multi-component reagent, thus a molarity is not available. When prepared according to instructions, the prepared solution will contain 0.288% (w/v) of sulfanilamide and 0.016% (w/v) naphthylethylene diaminedihydrochloride. In addition, the product also includes an unreported amount of phosphoric acid.
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How many grams of reagent powder should be dissolved in 1 ml of distilled water?
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This product is tested for solubility at 40 mg/mL in water. The product is intended to be reconstituted in the bottle by adding 250 ml of water. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/355/046/g4410bul.pdfHelpful?
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Since this is a solid, how much quantity of water it should be diluted with?
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The 10 g quantity is packaged in a 250 mL bottle. To reconstitute, simply add 250 mL of distilled water.
Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/355/046/g4410bul.pdfHelpful?
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how the limit of detection ?
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According to T.P. Misko, et al., Analytical Biochemistry, 214, 11-16 (1993), this Griess Reagent, in fluorometric testing, detects to at least 10 nM nitrite. This information has not been validated.
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What is advised for disposal if a solution is made up with H2O?
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Please refer to the SDS for disposal information. Per section 13.1, Waste material must be disposed of in accordance with the national and loc No mixing with other waste. Handle uncleaned containers like the product. See www.retrologistik.com for processes regarding the return of chemicals and containers, or contact technical service if you have further questions. https://www.sigmaaldrich.com/sds/sigma/g4410.
Please contact your EHS team or or disposal service group, If there's none, we recommend a national disposal company such as Cleanharbors at https://www.cleanharbors.com/.
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Is there an enzymatic method to convert nitrate to nitrite?
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A coupled assay to convert nitrate to nitrite has been published by Grisham, M.B., et al., Quantitation of nitrate and nitrite in extracellular fluids. Methods Enzymol., 268, 237-246 (1996). For this protocol, we recommend using Product No. N7265 (Nitrate Reductase), Product No.L1378 (L-Lactic Dehydrogenase), Product No.N1630 (NADPH), Product No. P8574 (Sodium pyruvate) and Product No. F6625 (Flavin adenine dinucleotide disodium salt).
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What is the difference between Product G4410 Griess reagent (modified), and Product 03553, Griess reagent?
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There are many formulations of the Griess Reagent. The formulations for these products are similar; both are proprietary.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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