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F4383
7-Fluorobenzofurazan-4-sulfonic acid ammonium salt
≥98%
Synonim(y):
4-Fluoro-7-sulfobenzofurazan ammonium salt, 7-Fluorobenz-2,1,3-oxadiazole-4-sulfonic acid ammonium salt, Ammonium 7-fluorobenzofurazan-4-sulfonate, SBD-F
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| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 10 mg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 3130,00 zł | |
| 25 mg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 3390,00 zł |
Informacje o tej pozycji
Wzór liniowy:
C6H3FN2O4S · NH3
Numer CAS:
Masa cząsteczkowa:
235.19
UNSPSC Code:
12352106
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
5199564
Assay:
≥98%
3130,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócQuality Level
assay
≥98%
storage condition
protect from light
fluorescence
λex 380 nm; λem 515 nm
storage temp.
−20°C
SMILES string
N.OS(=O)(=O)c1ccc(F)c2nonc12
InChI
1S/C6H3FN2O4S.H3N/c7-3-1-2-4(14(10,11)12)6-5(3)8-13-9-6;/h1-2H,(H,10,11,12);1H3
InChI key
JXLHNMVSKXFWAO-UHFFFAOYSA-N
General description
7-Fluorobenzofurazan-4-sulfonic acid ammonium salt, also known as SBD-F, is a low molecular weight sensitive fluorescent probe. SBD-F reacts with sulfhydryl groups (disulfides do not react with SBD-F and must first be reduced to thiols, such as tributylphosphine) to produce highly fluorescent compounds. The rate of reaction of thiols with SBD-F gradually increases with increasing pH. High fluorescence intensities are observed at pH 2-12. SBD-F has an excitation range between 380-385nm and an emission range between 510-515nm.
Application
Fluorescent probe for thiols and pre-column labeling of biological thiols for HPLC
SBD-F is suitable for the HPLC determination of biological thiols at the picomole level. SBD-F has been used in HPLC methods for measuring total plasma and serum homocysteine levels. It is also suitable to determine metallothionein in a tandem column HPLC method with an isocratic solvent system. SBD-F has been used for determining the position of disulfide linkages of cysteine residues in proteins and for detecting cysteine-containing peptides.
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Ta pozycja | |||
|---|---|---|---|
| assay ≥98% | assay ≥98.5% (HPLC) | assay - | assay >98% |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. room temp |
| fluorescence λex 380 nm; λem 515 nm | fluorescence λex 385 nm; λem 524 nm in 0.1 M borate pH 9.5 (after derivatization with glutathione) | fluorescence - | fluorescence λex 360 nm; λem 410 nm (pH 8.5), λex 383 nm; λem 528 nm (Reaction product) |
| storage condition protect from light | storage condition - | storage condition - | storage condition - |
Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Powiązane treści
Product Information Sheet
N P Dudman et al.
Clinical chemistry, 42(12), 2028-2032 (1996-12-01)
The recognition of homocysteine as a vascular risk factor has led to increased clinical interest in assaying plasma homocysteine concentrations. Our aim was to improve the reliability of a widely used assay based on HPLC of the fluorescent 7-benzo-2-oxa-1, 3-diazole-4-sulfonic
B L Ling et al.
Journal of pharmaceutical and biomedical analysis, 10(10-12), 985-988 (1992-10-01)
The present investigation analyses the potentials of capillary chromatography using packed fused silica capillaries filled with 5 microns RP-18 for the fluorescence determination of glutathione in human blood samples. Adaptation of conventional HPLC equipment for miniaturized chromatographic assays proved successful.
T Toyo'oka et al.
Biomedical chromatography : BMC, 3(4), 166-172 (1989-07-01)
Biological thiols and disulfides in rat and hamster tissues were simultaneously determined by HPLC-fluorescence detection using 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (ABD-F) and ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F). The coefficients of variation (CV) of the method for reduced glutathione (GSH) and oxidized glutathione (GSSG) in liver
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| F4383-1MG | 04061833617700 |
| F4383-25MG | 04061833617717 |
| F4383-10MG | 04061832534688 |