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Merck

F4383

7-Fluorobenzofurazan-4-sulfonic acid ammonium salt

≥98%

Synonim(y):

4-Fluoro-7-sulfobenzofurazan ammonium salt, 7-Fluorobenz-2,1,3-oxadiazole-4-sulfonic acid ammonium salt, Ammonium 7-fluorobenzofurazan-4-sulfonate, SBD-F

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Wybierz wielkość

10 MG

3130,00 zł

25 MG

3390,00 zł

3130,00 zł

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Informacje o tej pozycji

Wzór liniowy:
C6H3FN2O4S · NH3
Numer CAS:
84806-27-9
Masa cząsteczkowa:
235.19
UNSPSC Code:
12352106
NACRES:
NA.32
PubChem Substance ID:
24894861
MDL number:
MFCD02682109
Beilstein/REAXYS Number:
5199564
Assay:
≥98%

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InChI

1S/C6H3FN2O4S.H3N/c7-3-1-2-4(14(10,11)12)6-5(3)8-13-9-6;/h1-2H,(H,10,11,12);1H3

SMILES string

N.OS(=O)(=O)c1ccc(F)c2nonc12

InChI key

JXLHNMVSKXFWAO-UHFFFAOYSA-N

assay

≥98%

storage condition

protect from light

fluorescence

λex 380 nm; λem 515 nm

storage temp.

−20°C

Quality Level

200

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Ta pozycja
46640F3639C1154
7-Fluorobenzofurazan-4-sulfonic acid ammonium salt ≥98%

Sigma-Aldrich

F4383

7-Fluorobenzofurazan-4-sulfonic acid ammonium salt

7-Fluorobenzofurazan-4-sulfonic acid ammonium salt derivatization grade (HPLC), LiChropur™, ≥98.5% (HPLC)

Supelco

46640

7-Fluorobenzofurazan-4-sulfonic acid ammonium salt

4-Fluoro-7-sulfamoylbenzofurazan

Sigma-Aldrich

F3639

4-Fluoro-7-sulfamoylbenzofurazan

4-Chloro-7-sulfobenzofurazan ammonium salt

Sigma-Aldrich

C1154

4-Chloro-7-sulfobenzofurazan ammonium salt

assay

≥98%

assay

≥98.5% (HPLC)

assay

-

assay

>98%

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

room temp

storage condition

protect from light

storage condition

-

storage condition

-

storage condition

-

fluorescence

λex 380 nm; λem 515 nm

fluorescence

λex 385 nm; λem 524 nm in 0.1 M borate pH 9.5 (after derivatization with glutathione)

fluorescence

-

fluorescence

λex 360 nm; λem 410 nm (pH 8.5), λex 383 nm; λem 528 nm (Reaction product)

General description

7-Fluorobenzofurazan-4-sulfonic acid ammonium salt, also known as SBD-F, is a low molecular weight sensitive fluorescent probe. SBD-F reacts with sulfhydryl groups (disulfides do not react with SBD-F and must first be reduced to thiols, such as tributylphosphine) to produce highly fluorescent compounds. The rate of reaction of thiols with SBD-F gradually increases with increasing pH. High fluorescence intensities are observed at pH 2-12. SBD-F has an excitation range between 380-385nm and an emission range between 510-515nm.

Application

Fluorescent probe for thiols and pre-column labeling of biological thiols for HPLC
SBD-F is suitable for the HPLC determination of biological thiols at the picomole level. SBD-F has been used in HPLC methods for measuring total plasma and serum homocysteine levels. It is also suitable to determine metallothionein in a tandem column HPLC method with an isocratic solvent system. SBD-F has been used for determining the position of disulfide linkages of cysteine residues in proteins and for detecting cysteine-containing peptides.
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Klasa składowania

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certyfikaty analizy (CoA)

Lot/Batch Number
0000518337
0000518337
0000486228
0000488468
0000488468

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

N P Dudman et al.
Clinical chemistry, 42(12), 2028-2032 (1996-12-01)
The recognition of homocysteine as a vascular risk factor has led to increased clinical interest in assaying plasma homocysteine concentrations. Our aim was to improve the reliability of a widely used assay based on HPLC of the fluorescent 7-benzo-2-oxa-1, 3-diazole-4-sulfonic
T Toyo'oka et al.
Biomedical chromatography : BMC, 3(4), 166-172 (1989-07-01)
Biological thiols and disulfides in rat and hamster tissues were simultaneously determined by HPLC-fluorescence detection using 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (ABD-F) and ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F). The coefficients of variation (CV) of the method for reduced glutathione (GSH) and oxidized glutathione (GSSG) in liver
B L Ling et al.
Journal of pharmaceutical and biomedical analysis, 10(10-12), 985-988 (1992-10-01)
The present investigation analyses the potentials of capillary chromatography using packed fused silica capillaries filled with 5 microns RP-18 for the fluorescence determination of glutathione in human blood samples. Adaptation of conventional HPLC equipment for miniaturized chromatographic assays proved successful.
I Fermo et al.
Journal of chromatography. B, Biomedical sciences and applications, 719(1-2), 31-36 (1998-12-30)
We have modified a high-performance liquid chromatographic (HPLC) procedure based on SBD-F (ammonium-7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate) pre-column derivatization to obtain an assay that is useful for routine clinical total plasma homocysteine (tHcy) analysis. The introduction of easily handled sodium borohydride instead of the
P E Cornwell et al.
Journal of chromatography, 617(1), 136-139 (1993-07-23)
A modification of a previously published method for analysis of total homocysteine in human serum is presented. The modification was implemented to allow use of a different derivatizing agent (i.e., 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonamide) which reacts much faster than the original derivatizing reagent
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