DN25
Deoxyribonuclease I from bovine pancreas
lyophilized powder, Protein ≥85 %, ≥400 Kunitz units/mg protein
Synonim(y):
Deoxyribonuclease, DNase I, Deoxyribonucleate 5′-oligonucleotido-hydrolase
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About This Item
Polecane produkty
pochodzenie biologiczne
bovine pancreas
Postać
lyophilized powder
aktywność właściwa
≥400 Kunitz units/mg protein
masa cząsteczkowa
~31 kDa
skład
Protein, ≥85%
metody
DNA extraction: suitable
rozpuszczalność
0.15 M NaCl: soluble 5.0 mg/mL, hazy
przydatność
suitable for molecular biology
Zastosowanie
diagnostic assay manufacturing
diagnostic assay manufacturing
obecność zanieczyszczeń
RNase ≤0.02%
Warunki transportu
wet ice
temp. przechowywania
−20°C
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Opis ogólny
DNase I, or Deoxyribonuclease I, is an endonuclease isolated from bovine pancreas.
- Our DNase I Digests double str and single stranded DNA into oligo and mononucleotides.
- Bovine pancreatic DNase exists as four isozymes, having isoelectric points for A, B, C and D: 5.22, 4.96, 5.06 and 4.78.3. The predominant form is A, with smaller amounts of B and C, and only minor amount of D.
- DNase I structure resembles the structure of to exonuclease III. It includes two central ß sheets. Each β sheet is composed of six β-strands. This complex of β sheets is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III.[1]
Zastosowanie
- Decreases viscosity providing better yields by removing DNA in primary cell isolation:
- Incorporating labelled bases into DNA: DNA nick
- Radioactive labelling
- Bioprocessing applications: DNA removal
- Eliminating genomic DNA from RNA preparations before RT-PCR
- In vitro transcription
- Nick translation
- DNase footprinting
- Actin regulation of actin polymerization in cells, and cell apoptosis
- UV crosslinking of proteins to nucleic acids
- DNase play a role in the regulation of actin polymerization in cells and is involved in apoptosis process [1]
Used for the removal of DNA from protein samples.
Działania biochem./fizjol.
DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. The pH optimum is found to be between 7 and 8. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1 respectively. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity.
Cechy i korzyści
Our Deoxyribonuclease DNAse I,is essentially RNAse free product, which support the product application.
Definicja jednostki
One Kunitz unit will produce a change in A260 of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III, as substrate.
Postać fizyczna
Crude preparation, contains calcium chloride
Uwaga dotycząca przygotowania
10 mg/mL solution of DNAse I in 0.15 M NaCl may lose <10% of its activity when stored for a week in aliquots at −20 °C. The same solutions stored in aliquots at 2-8 °C can lose approximately 20% activity. It remains active for up to five hours at 60 °C between pH 5 and 7, and loses activity in <10 minutes at 68 °C. It loses activity at the rate of 6%/hour in acetate buffer (pH 5.0) and tris buffer ((pH 7.2) at 1 mg/mL concentration.
Komentarz do analizy
Protein determined by biuret.
inhibitor
Numer produktu
Opis
Cennik
Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Resp. Sens. 1
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
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Protokoły
Standaryzacja procedury enzymatycznego oznaczania deoksyrybonukleazy I.
To standardize a procedure for the enzymatic assay of Deoxyribonuclease I.
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