260913

DNase I, Bovine Pancreas

Native DNase I from bovine pancreas.

• Synonim(y): DNase I, Bovine Pancreas, Deoxyribonuclease I
• Numer CAS: 9003-98-9
• Numer WE: 3.1.21.1 (BRENDA, IUBMB)
• UNSPSC Code: 12352202
• NACRES: NA.54
• MDL number: MFCD00130918
• Specific activity: ≥60,000 dornase units/mg dry wt
• Biological source: bovine pancreas

Właściwości

• biological source: bovine pancreas
• Quality Segment: 200
• description: Merck USA index - 14, 2906
• form: lyophilized
• specific activity: ≥60,000 dornase units/mg dry wt
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated
• solubility: sodium acetate buffer: soluble, water: soluble
• shipped in: ambient
• storage temp.: −20°C

Opis

General description

Native DNase I from bovine pancreas. An endonuclease that preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides to yield 5′-phosphate terminated polynucleotides with a free hydroxyl group at the 3′ position. Acts on single and double stranded DNA. Note: 1 MU = 1,000,000 Dornase units

Note: 32 Dornase units equal ~1 Kunitz unit; 1 MU = 1,000,000 Dornase units.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

One Dornase unit is defined as the amount of enzyme that will cause a decrease of 1.0 relative viscosity unit in a solution of highly polymerized DNA from the initial relative viscosity of 4.0 in 10 min at 30°C.

Takeshita, H., et al. 2000. Biochem. Biophys. Res. Commun.269, 481.
Jones, S.J., et al. 1996. J. Mol. Biol.264, 1154.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Informacja o środkach bezpieczeństwa

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable