50937

Guanidine hydrochloride solution

BioUltra, ~8 M in H₂O

• Synonim(y): Aminoformamidine hydrochloride, Guanidinium chloride, Guanidium chloride
• Wzór empiryczny (zapis Hilla): CH₅N₃ · HCl
• Numer CAS: 50-01-1
• Masa cząsteczkowa: 95.53
• UNSPSC Code: 12352107
• NACRES: NA.25
• PubChem Substance ID: 57651286
• MDL number: MFCD00013026
• Beilstein/REAXYS Number: 3591990

Właściwości

• product line: BioUltra
• Quality Segment: 100
• form: liquid
• concentration: ~8 M in H₂O
• impurities: insoluble matter, passes filter test
• refractive index: n20/D 1.465
• pH: 4.5-7.5 (25 °C)
• anion traces: sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Al: ≤1 mg/kg, As: ≤0.1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤10 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤5 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Na: ≤150 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg
• λ: neat
• UV absorption: λ: 260 nm A_max: 0.040, λ: 280 nm A_max: 0.025
• application(s): advanced drug delivery
• SMILES string: Cl.NC(N)=N
• InChI: 1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H
• InChI key: PJJJBBJSCAKJQF-UHFFFAOYSA-N
• Gene Information: human ... KCNA1(3736), KCNA10(3744), KCNA2(3737), KCNA3(3738), KCNA4(3739), KCNA5(3741), KCNA6(3742), KCNA7(3743), KCNB1(3745), KCNB2(9312), KCNC1(3746), KCNC2(3747), KCNC3(3748), KCNC4(3749), KCND1(3750), KCND2(3751), KCND3(3752), KCNF1(3754), KCNG1(3755), KCNG2(26251), KCNG3(170850), KCNG4(93107), KCNH1(3756), KCNH2(3757), KCNH3(23416), KCNH4(23415), KCNH5(27133), KCNH6(81033), KCNH7(90134), KCNH8(131096), KCNQ1(3784), KCNQ2(3785), KCNQ3(3786), KCNQ4(9132), KCNQ5(56479), KCNS1(3787), KCNS2(3788), KCNS3(3790), KCNV1(27012), KCNV2(169522)

Opis

Application

Guanidine hydrochloride solution has been used as a fixation buffer in genomic DNA extraction for genomic analysis.

Biochem/physiol Actions

Guanidine‐HCl (Gnd-HCl), an efficient chaotropic agent, is broadly used in RNA isolation, globular protein denaturation, and protein refolding studies as a denaturant. It is an appropriate sample buffer for the quick and effective elution of affinity-purified protein complexes and the subsequent protein digestion step.[1] Gnd-HCl is not preferred in proteomics experiments due to its hindrance with trypsin activity at low molar concentrations and tendency to precipitate when combined with sodium dodecyl sulfate (SDS).[1] GnHCl is commonly utilized to extract proteins in extracellular matrix (ECM)‐rich tissues including bone, cartilage, ligament, and tendon.[2]

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Informacja o środkach bezpieczeństwa

• pictograms: GHS07
• signalword: Warning
• hcodes: H302 + H332,H315,H319
• pcodes: P261 - P264 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
• Klasa składowania: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter