43815

DL-Dithiothreitol

BioUltra, for molecular biology, ≥99.5% (RT)

• Synonim(y): (±)-Dithiothreitol, rac-Dithiothreitol, Dithiothreitol, threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT
• Wzór liniowy: HSCH₂CH(OH)CH(OH)CH₂SH
• Numer CAS: 3483-12-3
• Masa cząsteczkowa: 154.25
• Beilstein: 1719757
• Numer WE: 222-468-7
• Numer MDL: MFCD00004877
• Kod UNSPSC: 12161504
• Identyfikator substancji w PubChem: 57650195
• NACRES: NA.25

Właściwości

• klasa czystości: for molecular biology
• linia produktu: BioUltra
• Próba: ≥99.5% (RT)
• Postać: powder
• przydatność reakcji: reagent type: reductant
• zanieczyszczenia: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected; ≤0.3% 4,5-dihydroxy-1,2-dithiane
• pH: 4.0-6.5 (25 °C, 0.1 M in H₂O)
• mp: 41-44 °C (lit.)
• rozpuszczalność: H₂O: 0.1 M at 20 °C, clear, colorless
• ślady anionów: sulfate (SO₄^2-): ≤50 mg/kg
• ślady kationów: Al: ≤5 mg/kg; As: ≤0.5 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤500 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 0.1 M in H₂O
• absorpcja UV: λ: 260 nm A_max: 0.400; λ: 280 nm A_max: 0.100
• ciąg SMILES: O[C@H](CS)[C@H](O)CS
• temp. przechowywania: 2-8°C
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
• Klucz InChI: VHJLVAABSRFDPM-QWWZWVQMSA-N

Opis

Opis ogólny

Dithiothreitol (DTT) is a thiol-reducing reagent that quantitatively reduces disulfide (SH) groups in biological molecules. It specifically targets the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and less toxic than 2-mercaptoethanol. Typically, a seven-fold lower concentration of DTT (100 mM) is more efficient than that of 2-mercaptoethanol (5% v/v, 700 mM).

Zastosowanie

DTT has been used:

• as an enzyme stabilizer to maintain exebacase stability
• for peptide extraction and protein denaturation
• in sample preparation and reversal of formaldehyde crosslinks before mass spectrometry
• as a buffer component to extract synaptogliosome

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Działania biochem./fizjol.

Dithiothreitol (DTT) is broadly used in chemical peptide synthesis and biochemical preparations of thiol proteins. Additionally, studies on protein folding and enzyme activity in protein chemistry make use of DTT. This reagent explicitly mediates the thiol-disulfide exchange reaction to completely reduce the intra-or between sub-atomic disulfide bonds in biomolecules. This reaction brings about the arrangement of thiols and the cyclic disulfide of DTT.

Cechy i korzyści

• BioUltra grade powder suitable for molecular biology
• DNase, RNase, phosphatase, and protease-free insoluble matter, passes the filter test
• Stringently tested and free from trace metals

Inne uwagi

For additional information on our range of Biochemicals, please complete this form.

Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds.

Informacja o środkach bezpieczeństwa

• Piktogramy: GHS05,GHS07
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H302,H315,H318
• Zwroty wskazujące środki ostrożności: P264 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Klasyfikacja zagrożeń: Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: dust mask type N95 (US), Eyeshields, Faceshields, Gloves