43819

DL-Dithiothreitol

≥99.0% (RT)

• Synonim(y): threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT
• Wzór liniowy: HSCH₂CH(OH)CH(OH)CH₂SH
• Numer CAS: 3483-12-3
• Masa cząsteczkowa: 154.25
• UNSPSC Code: 12352201
• NACRES: NA.21
• PubChem Substance ID: 329756409
• EC Number: 222-468-7
• Beilstein/REAXYS Number: 1719757
• MDL number: MFCD00004877

Właściwości

• Quality Segment: 200
• assay: ≥99.0% (RT)
• form: powder
• reaction suitability: reagent type: reductant
• impurities: ≤0.3% 4,5-dihydroxy-1,2-dithiane
• mp: 40-43 °C, 41-44 °C (lit.)
• solubility: H₂O: 50 mg/mL, clear, colorless, H₂O: freely soluble, acetone: freely soluble, chloroform: freely soluble, diethyl ether: freely soluble, ethanol: freely soluble, ethyl acetate: freely soluble
• anion traces: sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Na: ≤250 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Zn: ≤5 mg/kg
• storage temp.: 2-8°C
• SMILES string: O[C@H](CS)[C@H](O)CS
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
• InChI key: VHJLVAABSRFDPM-QWWZWVQMSA-N

Opis

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

DL-Dithiothreitol is a pivotal component used in the preparation of buffer which in turn is utilized in the platelet cytosol preparation.[1] It is also important in isolation of RNA by using guanidinium salts and homogenisation of tissue[2] and cells[3].

Biochem/physiol Actions

DL-Dithiothreitol denatures proteins by reducing the disulphide bonds and reducing them to SH groups. It also prevents intramolecular and intermolecular disulfide bond formation between cysteine residues of proteins.[4]

Other Notes

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Informacja o środkach bezpieczeństwa

• pictograms: GHS05,GHS07
• signalword: Danger
• hcodes: H302,H315,H318
• pcodes: P264 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves