43816

DL-Dithiothreitol solution

BioUltra, for molecular biology, ~1 M in H₂O

• Synonim(y): Cleland′s reagent, (±)-Dithiothreitol, rac-Dithiothreitol, Dithiothreitol, (±)-threo-1,4-Dimercapto-2,3-butanediol solution, DTT
• Wzór empiryczny (zapis Hilla): C₄H₁₀O₂S₂
• Numer CAS: 3483-12-3
• Masa cząsteczkowa: 154.25
• Beilstein: 1719757
• Numer MDL: MFCD00004877
• Kod UNSPSC: 12352201
• Identyfikator substancji w PubChem: 57650196
• NACRES: NA.25

Właściwości

• klasa czystości: for molecular biology
• Poziom jakości: 200
• linia produktu: BioUltra
• Próba: 900-1100 mmol/L (Titration molarity)
• Postać: liquid
• przydatność reakcji: reagent type: reductant
• stężenie: ~1 M in H₂O
• zanieczyszczenia: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• pH: 2.7-5.0
• gęstość: 1.04 g/mL at 20 °C
• absorpcja: cut-off at 330 nm
• przydatność: in accordance for filter test; suitable for molecular biology
• temp. przechowywania: 2-8°C
• ciąg SMILES: O[C@H](CS)[C@H](O)CS
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
• Klucz InChI: VHJLVAABSRFDPM-QWWZWVQMSA-N

Opis

Opis ogólny

Dithiothreitol (DTT), a thiol group protectant, is an excellent reagent for preventing the oxidation of SH groups in proteins. DTT solution is a potent and versatile disulfide-reducing agent. It reduces a disulfide bond by forming a six-membrane ring and initiating a thiol-disulfide interchange.

Zastosowanie

DL-dithiothreitol solution has been used:

• in the treatment of intestinal explants
• in homogenate preparation of gill cells for enzymatic assays
• in protein electrophoresis for western blotting
• in transglutaminase activity
• in sample buffer for SDS-PAGE
• in Western Blot, as a lysis buffer component for single-nucleus RNA-sequencing
• as a component of protein extraction buffer for cell lysis

Działania biochem./fizjol.

DL-dithiothreitol (DTT) is used in a chemical debonding technique, that helps to remove bacteria from biofilms. It can change the structural stability of the GABA(B) (gamma-aminobutyric acid) receptor by disabling the disulfide bonds between four cysteine residues positioned in the GABA(B1(a)) receptor structure.Dithiothreitol (DTT) is broadly utilized in synthetic peptide synthesis and biochemical arrangements of thiol proteins. It can also be used to study protein chemistry topics like enzyme activity and protein folding. DTT explicitly controls the thiol-disulfide exchange response to completely reduce the intra-or between sub-atomic disulfide bonds in biomolecules. Thiols and the cyclic disulfide of DTT are the products of this reaction.

Cechy i korzyści

• BioUltra grade - 1M DTT solution, suitable for molecular biology
• Free from DNases, RNases, phosphatases, and proteases

Inne uwagi

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Informacja o środkach bezpieczeństwa

• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter