P3504

Ponceau S

Powder

• Synonim(y): 3-Hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt
• Wzór empiryczny (zapis Hilla): C₂₂H₁₂N₄Na₄O₁₃S₄
• Numer CAS: 6226-79-5
• Masa cząsteczkowa: 760.57
• NACRES: NA.47
• PubChem Substance ID: 24898418
• UNSPSC Code: 12171500
• EC Number: 228-319-2
• MDL number: MFCD00003892
• Colour Index Number: 27195

Właściwości

• Nazwa produktu: Ponceau S, BioReagent, suitable for electrophoresis
• product line: BioReagent
• Quality Segment: 200
• form: powder
• technique(s): electrophoresis: suitable
• color: red to very dark red
• solubility: H₂O: 1 mg/mL
• ε (extinction coefficient): ≥330000 at 518-522 nm in deionized water at 0.015 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O
• InChI: 1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;
• InChI key: KQHKSGRIBYJYFX-WNDMICSESA-J

Opis

General description

Ponceau S is the sodium salt of a diazo dye. It is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. The stain is reversed with water washes and aids in subsequent immunological detection.
The following are two common stain formulations:
0.1% Ponceau S (w/v) in 5% acetic acid
2% Ponceau S (w/v) in 30% TCA, 30% sulfosalicylic acid

Application

Ponceau S has been used for membrane staining (Western blotting.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves