Ponceau S is not recommended for gel staining; it is advised only for use with membranes. Using Ponceau S in acetic acid may precipitate and fix the proteins in the gel, which is not advisable if a protein transfer to a membrane is planned for a western blot.
Przejdź do
P7170
Ponceau S solution
BioReagent, 0.1 % (w/v) in 5% acetic acid
Synonim(y):
Ponceau S
Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.
Wybierz wielkość
Zmień widok
| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 1 L | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 965,00 zł |
Informacje o tej pozycji
Wzór empiryczny (zapis Hilla):
C22H12N4Na4O13S4
Numer CAS:
Masa cząsteczkowa:
760.57
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
MDL number:
965,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomócNazwa produktu
Ponceau S solution, BioReagent, suitable (for use in cellulose acetate electrophoresis), 0.1 % (w/v) in 5% acetic acid
description
for protein analysis
Quality Segment
product line
BioReagent
form
liquid
concentration
0.1 % (w/v) in 5% acetic acid
technique(s)
western blot: suitable
density
1.01 g/cm3 at 20 °C
suitability
suitable (for use in cellulose acetate electrophoresis), suitable for electrophoresis
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O
InChI
1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;
InChI key
KQHKSGRIBYJYFX-WNDMICSESA-J
General description
Ponceau S is the sodium salt of a diazo dye. Ponceau S solution is a suitable reagent for use in electrophoresis studies. The Ponceau S stain comes ready-to-use and is designed for rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It may be employed as stain for the purified fibrinolytic enzyme (CK) for the analysis of N-terminal amino acid sequence of the first 14 residues of CK by a protein sequencer. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1M NaOH for 1 minute. The stained and destained membrane can then be immunologically detected (Western blotting). A product information sheet is supplied which includes the staining procedure for cellulose acetate, nitrocellulose, and PVDF.
Application
Ponceau S solution has been used for membrane staining (Western blotting).
Ta strona może zawierać tekst przetłumaczony maszynowo.
1 of 1
Ta pozycja | |||
|---|---|---|---|
| technique(s) western blot: suitable | technique(s) microbe id | staining: suitable | technique(s) electrophoresis: suitable | technique(s) titration: suitable |
| suitability suitable (for use in cellulose acetate electrophoresis), suitable for electrophoresis | suitability - | suitability - | suitability - |
| concentration 0.1 % (w/v) in 5% acetic acid | concentration - | concentration - | concentration 0.04 wt. % in H2O |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| form liquid | form powder | form powder | form liquid |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. room temp |
Still not finding the right product?
Explore all of our products under Ponceau S solution
Klasa składowania
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Protokoły
Protokół Western blot szczegółowo opisuje przenoszenie białek z żeli na nitrocelulozę, co ma kluczowe znaczenie dla procedur immunoblottingu w badaniach naukowych.
Western blot protocol details protein transfer from gels to nitrocellulose, crucial for immunoblotting procedures in research.
Produkty
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
Powiązane treści
Joshua P Nederveen et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(7), 8975-8989 (2020-05-29)
Skeletal muscle satellite cell (SC) function and responsiveness is regulated, in part, through interactions within the niche, in which they reside. Evidence suggests that structural changes occur in the SC niche as a function of aging. In the present study
Immunomodulatory effect of arsenic on cytokine and HSP gene
expression in Labeo rohita fingerlings
expression in Labeo rohita fingerlings
Sudeshna Banerjee
Fish & Shellfish Immunology, 44 (2015)
Legume Grains Enhance Ileal Losses of Specific Endogenous Serine-Protease Proteins in Weaned Pigs1
Paulo Salgado
The Journal of Nutrition (2002)
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| P7170-30ML | 04061837280764 |
| P7170-1L | 04061838262110 |
-
Is it practical to stain protein gels directly with Ponceau S without transferring to membranes in order to assess the amount of protein and number of bands in a protein sample? Is there a direct gel staining protocol available for this purpose?
1 answer-
Helpful?
-