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Dokumenty

A7431

Sigma-Aldrich

Agarose

BioReagent, for molecular biology, Wide range/Standard 3:1

Synonim(y):

3,6-Anhydro-α-L-galacto-β-D-galactan

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About This Item

Numer CAS:
9012-36-6
Numer WE:
232-731-8
Numer MDL:
MFCD00081294
Kod UNSPSC:
41105317
Identyfikator substancji w PubChem:
24891233
NACRES:
NA.25

pochodzenie biologiczne

algae (marine)

Poziom jakości

200

klasa czystości

for molecular biology

linia produktu

BioReagent

Postać

powder

metody

electrophoresis: suitable

EEO

≤0.13

mp

≤90 °C

temp. przejścia

gel point 32.5-38 °C (4% gel)

siła żelu

≥1200 g/cm2 (4% gel)

ślady anionów

sulfate (SO42-): ≤0.15%

obecność zanieczyszczeń

DNase, RNase, NICKase, none detected

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

Klucz InChI

MJQHZNBUODTQTK-WKGBVCLCSA-N

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Zastosowanie

Specially formulated to form strong, flexible gels for separation of small (≤1 kb) PCR products, DNA and RNA. Stronger gels make gel-handling easier. Sharp resolution is produced with DNA fragments ranging from 10-1500 bp.

Komentarz do analizy

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)

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Certyfikaty analizy (CoA)

Lot/Batch Number
0000374470
0000374470
SLCJ6994
SLCJ0417
SLCB6757

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