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Merck

A2790

Sigma-Aldrich

Agarose

Wide range, for molecular biology

Synonim(y):

3,6-Anhydro-α-L-galacto-β-D-galactan

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About This Item

Numer CAS:
Numer WE:
Numer MDL:
Kod UNSPSC:
41105317
Identyfikator substancji w PubChem:
NACRES:
NA.25

pochodzenie biologiczne

algae (marine)

Poziom jakości

klasa czystości

for molecular biology

Postać

powder

metody

electrophoresis: suitable

EEO

≤0.15

mp

≤65 °C

temp. przejścia

gel point ≤35 °C

ślady anionów

sulfate (SO42-): ≤0.15%

obecność zanieczyszczeń

DNase, RNase, none detected

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

Klucz InChI

MJQHZNBUODTQTK-WKGBVCLCSA-N

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Zastosowanie

Wide range agarose is capable of separating DNA fragments from 50-1,000 bp on a single 3% agarose gel.

Komentarz do analizy

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


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