63069

Magnesium chloride solution

BioUltra, Molecular Biology, ~1 M in H₂O

• Synonim(y): Magnesium Chloride hydrate, Magnesium chloride (MgCl₂)hexahydrate, MgCl₂
• Wzór liniowy: MgCl₂
• Numer CAS: 7786-30-3
• Masa cząsteczkowa: 95.21
• NACRES: NA.26
• PubChem Substance ID: 329759772
• UNSPSC Code: 12352302
• MDL number: MFCD00011106
• Grade: Molecular Biology
• Form: liquid
• Solubility: water: soluble

Właściwości

• grade: Molecular Biology
• Quality Segment: 200
• product line: BioUltra
• form: liquid
• concentration: ~1 M in H₂O
• technique(s): HPLC: suitable, flow cytometry: suitable
• impurities: DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.0002% total Nitrogen (N)
• pH: 5.0-7.5 (25 °C, 1 M in H₂O)
• solubility: water: soluble
• density: 1.08 g/mL at 20 °C
• anion traces: phosphate (PO₄^3-): ≤10 mg/kg, sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Al: ≤1 mg/kg, As: ≤0.1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤10 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤50 mg/kg, Li: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Na: ≤500 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg
• λ: 1 M in H₂O
• UV absorption: λ: 260 nm A_max: 0.05, λ: 280 nm A_max: 0.03
• suitability: suitable for molecular biology
• application(s): cell analysis; filtration; vaccine development
• SMILES string: Cl[Mg]Cl
• InChI: 1S/2ClH.Mg/h2*1H;/q;;+2/p-2
• InChI key: TWRXJAOTZQYOKJ-UHFFFAOYSA-L

Opis

General description

Magnesium chloride is widely used in polymerase chain reactions (PCR) buffers to supply magnesium ions. The concentration of Mg²⁺ is the most critical parameter that impacts both the efficiency and specificity of PCR when total genomic DNA is used, synthesis of long products, or during multiplex reactions. Elevated concentrations of Mg²⁺ enhance the stability of primer-template duplexes and the efficiency of amplification.[1]

Application

Magnesium chloride solution has been used in the reaction mix of reverse transcriptase enzyme, PFV RT. It has been used in the preparation of artificial cerebrospinal fluid (ACSF).[2]

Magnesium chloride solution has been used as a component of nuclei isolation buffer for in situ fixations and isolation of nuclei from Kupffer cells (or other liver cells).[3]

Other Notes

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• Klasa składowania: 12 - Non Combustible Liquids
• ppe: Eyeshields, Gloves