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410217
2′,7′-Dichlorofluorescein
ACS reagent
Synonim(y):
Dichlorofluorescein; Fluorescein 27
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| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 1 g | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 406,00 zł | |
| 10 g | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 2180,00 zł |
Informacje o tej pozycji
Wzór empiryczny (zapis Hilla):
C20H10Cl2O5
Numer CAS:
Masa cząsteczkowa:
401.20
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
200-968-6
Beilstein/REAXYS Number:
58009
MDL number:
406,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
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ACS reagent
Quality Segment
form
powder
technique(s)
titration: suitable
color
orange to red-brown, powder
mp
280 °C (dec.) (lit.)
solubility
alcohol: passes test
density
0.790 g/cm3
λmax
509 nm
suitability
passes test for adsorption indicator
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl
InChI
1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
InChI key
VFNKZQNIXUFLBC-UHFFFAOYSA-N
General description
2′,7′-Dichlorofluorescein is an oxidation-sensitive fluorescent probe.
Application
2′,7′-Dichlorofluorescein has been used to measure ROS (reactive oxygen species) formation in cells. It has been used as a fluorogenic substrate to study the peroxidase activity of saliva samples and extracts obtained from submandibular glands.
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Ta pozycja | |||
|---|---|---|---|
| technique(s) titration: suitable | technique(s) titration: suitable | technique(s) - | technique(s) - |
| suitability passes test for adsorption indicator | suitability - | suitability - | suitability - |
| grade ACS reagent | grade - | grade - | grade - |
| color orange to red-brown, powder | color - | color - | color - |
| form powder | form powder | form solid | form powder |
| solubility alcohol: passes test | solubility ethanol: 25 mg/mL | solubility - | solubility - |
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Klasa składowania
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| 410217-10G | 04061832011332 |
| 410217-1G | 04061832011356 |
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What is the Department of Transportation shipping information for this product?
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In what types of applications can 2´,7´-Dichlorofluorescein be used?
1 answer-
Some applications for use of 2´,7´-Dichlorofluorescein are listed below. This product can be used for the determination of carbohydrates by fluorescence densitometry after TLC. See G. Gübitz et al., J. Chromatogr., 117, 337, (1976). 2´,7´-Dichlorofluorescein as also been used in the determination of H2O2 by chemiluminescence. See the following references by H. Watanabe et al. Anal. Sci. 2, 461, (1986) and T. Segawa et al. Anal. Sci. 6, 763, (1990). This product has also been used as a fluorescent additive used in indirect fluorimetric detection in OTC-HPLC. See W.D. Pfeffer and E.S. Yeung, J. Chromatogr., 506, 401, (1990).
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What excitation /emission wavelengths are best for fluorescence detection of 2´,7´-Dichlorofluorescein?
1 answer-
2´,7´-Dichlorofluorescein can be detected at an excitation wavelength at 504 nm and emission wavelength at 529 nm in 0.1 M Tris pH 8.0.
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What can be used to make a solution of 2´,7´-dichlorofluorescein?
1 answer-
2´,7´-Dichlorofluorescein is soluble at 25 mg/mL absolute ethanol with heating. 2´,7´-Dichlorofluorescein is also soluble in water at 0.1 mg/mL.
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