MABF81

Anti-ROR gamma T Antibody, clone 6F3.1

clone 6F3.1, from mouse

• Synonim(y): Nuclear receptor ROR-gamma, Nuclear receptor RZR-gamma, Nuclear receptor subfamily 1 group F member 3, Retinoid-related orphan receptor-gamma
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 6F3.1, monoclonal
• Application: IHC, WB
• Citations: 21

Właściwości

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 6F3.1, monoclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable, western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_001001523
• UniProt accession no.: P51449
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... RORC (6097)

Opis

General description

Retinoid-related orphan receptor-gamma t (RORγT) is one of two RORγ isoforms belonging to the nuclear hormone receptor family and contains a single nuclear receptor DNA-binding domain. RORγT is a negative regulator of TNFDF6/FASL expression and T-cell production of IL2. The upregulation of RORγT is a requirement for TH17 cell differentiation. Inhibition of RORγT is induced by TGF-β’s interaction with Foxp3. Expression is observed mainly in immature thymocytes, and, during fetal development, is found exclusively in lymphoid-inducer (LTi) cells.

~55 kDa observed. Two isoforms at ~55 kDa and ~58 kDa may be observed in some cell lysates.

Immunogen

GST-tagged recombinant protein corresponding to human ROR gamma T.

Application

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected ROR gamma T in normal human liver tissues.

Research Category
Inflammation & Immunology

Research Sub Category
Inflammation & Autoimmune Mechanisms

This Anti-ROR gamma T Antibody, clone 6F3.1 is validated for use in WB, IH for the detection of ROR gamma T.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
T47D cell lysate

Evaluted in Western Blot in T47D cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected ROR gamma T in 10 µg of T47D cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable