MABS496

Anti-Mineralocorticoid Receptor Antibody, clone 6G1

clone 6G1, from mouse

• Synonim(y): MR, Nuclear receptor subfamily 3 group C member 2, Mineralocorticoid Receptor
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 6G1, monoclonal
• Application: ICC, IHC, WB
• Citations: 5

Właściwości

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 6G1, monoclonal
• species reactivity: rat, mouse
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG1
• NCBI accession no.: NP_037263
• UniProt accession no.: P22199
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Nr3C2(110784)

Opis

General description

Mineralocorticoid receptor (UniProt P22199; also known as MR, Nuclear receptor subfamily 3 group C member 2) is encoded by the Nr3c2 (also known as MCR, Mlr) gene (Gene ID 25672) in rats. Mineralocorticoid receptor (MR) is expressed in multiple tissues, including renal epithelia, smooth muscle, endothelium, cardiomyocytes, and hippocampal neurons, where it mediates diverse functions. MR is activated by aldosterone and also by cortisol in cells that do not express 11β-hydroxysteroid dehydrogenase type 2 (11βHSD2). MR is normally activated by aldosterone, which is produced by adrenal glomerulosa in response to intravascular volume depletion and hyperkalemia. Consistently, gain of function mutations in MR lead to severe hypertension, often with hypokalemia, while loss of function mutations result in neonatal hypotension. Ser843 phosphorylation in the MR ligand-binding domain is reported to prevent MR ligand binding and activation. MR pS843 is found exclusively in intercalated cells of the distal nephrons in the kidney. Intravascular volume depletion, angiotensin II and WNK4 signaling reduce MR pS843 levels, whereas hyperkalemia increases MR pS843.

~107 kDa observed

Immunogen

Conjugated linear peptide corresponding to rat Mineralocorticoid Receptor.

Application

Detect Mineralocorticoid Receptor using this Anti-Mineralocorticoid Receptor Antibody, clone 6G1 validated for use in Western Blotting, Immunohistochemistry and Immunocytochemistry.

Immunohistochemistry Analysis: A representative lot detected Mineralocorticoid Receptor in various rat tissues including kidney, hippocampus, choroid plexus, cerebellum, colon, heart, and coronary artery (Gomez-Sanchez, C.E., et al. (2006). Endocrinology. 147(3):1343-1348).
Immunocytochemistry Analysis: A representative lot detected Mineralocorticoid Receptor in mouse kidney cells (Shibata, S., et al. (2013). Cell Metabolism. 18:660-671).
Western Blotting Analysis: A representative lot detected Mineralocorticoid Receptor in rat hippocampal tissue lysate (Gomez-Sanchez, C.E., et al. (2006). Endocrinology. 147(3):1343-1348).
Western Blotting Analysis: A representative lot detected endogenous Mineralocorticoid Receptor (MR) in mouse kidney and exogenously expressed human MR in transfected COS-7 cells (Shibata, S., et al. (2013) Cell Metab. 18(5):660-671).

Biochem/physiol Actions

Epitope is present in all 3 alternative spliced isoforms.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in M1MR cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Mineralocorticoid Receptor in 10 µg of M1MR cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable