MAB4703

Anti-Caspase 3 Antibody, large subunit & proform, clone 4-1-18

clone 4-1-18, Chemicon^®, from mouse

• Synonim(y): CPP32
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 4-1-18, monoclonal
• Application: IHC, WB
• Citations: 9

Właściwości

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 4-1-18, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NM_004346.3, NM_032991.2
• UniProt accession no.: P42574
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CASP3(836)

Opis

Immunogen

Epitope: large subunit & proform

Recombinant full-length human caspase-3 protein

Application

Anti-Caspase 3 Antibody, large subunit & proform, clone 4-1-18 detects level of Caspase 3 & has been published & validated for use in WB, IH(P).

Research Category
Apoptosis & Cancer

Metabolism

Research Sub Category
Caspases

Enzymes & Biochemistry

Western blot: 1:1000. The antibody will detect a band at 32 kDa corresponding to the inactive unprocessed form of caspase 3 as well as a 17-22 kDa band corresponding to the large subunit of active caspase 3.

Immunohistochemistry (frozen and paraffin): 1:500.



Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Recognizes the full length 32 kDa caspase-3 protein. Highly specific to caspase-3 and shows no cross-reaction with other caspase family members. Caspase-3 is ubiquitously expressed and like other caspases is synthesized as an inactive proenzyme. Upon activation, caspase-3 is cleaved generating two smaller subunits of 17 kDa and 12 kDa. Caspase-3 is one of the most extensively studied enzyme in apoptosis. Activation of caspase-3 occurs in response to a variety of apoptotic inducers.

Physical form

Format: Purified

Purified immunoglobulin by protein A. Liquid in PBS, pH 7.6, with 0.1% sodium azide.

Preparation Note

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Analysis Note

Control
POSITIVE CONTROL: 293 (human embryonic kidney) cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-439

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• Klasa składowania: 12 - Non Combustible Liquids