MAB10753

Anti-Caspase3 (active form) Antibody, clone 3D9.3

clone 3D9.3, from mouse

• Synonim(y): Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, SCA-1, Caspase-3 subunit p17, Caspase-3 subunit p12
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 3D9.3, monoclonal
• Application: FACS, ICC, WB
• Citations: 7

Właściwości

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 3D9.3, monoclonal
• species reactivity: human
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable, western blot: suitable
• isotype: IgMκ
• NCBI accession no.: NP_004337
• UniProt accession no.: P42574
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CASP3(836)

Opis

General description

Caspase-3 (CPP32, Apopain) is the most extensively studied apoptotic protein. Caspase-3 is synthesized as an inactive proenzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells such as PARP and DFF.

~17 kDa observed. Caspase3 is cleaved during apoptosis into 17 kDa and 12 kDa active forms.

Immunogen

KLH-conjugated linear peptide corresponding to human Caspase3 (active form).

Application

Anti-Caspase3 (active form), clone 3D9.3 is an antibody targeting the Caspase3 (active form) protein, validated for use in WB, ICC & Flow.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Caspase3 (active form) in Hela and Hela/staurosporine 3 hr cells.
Flow Cytometry Analysis: A 1:400 dilution from a representative lot detected Caspase3 (active form) in Jurkat T cells.

Biochem/physiol Actions

This antibody demonstrates specificity for the cleaved 17 kDa active form, but does not cross-react with the non-active (pro) form Caspase3 at 35 kDa.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Caspase3 (active form) in 10 µg of HeLa and staurosporine treated HeLa cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-1090; 04-439

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• WGK: WGK 2