MAB030
Anti-DNA Antibody, double stranded, clone BV16-13
culture supernatant, clone BV16-13, Chemicon®
Synonim(y):
Double-stranded DNA antibody
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
culture supernatant
rodzaj przeciwciała
primary antibodies
klon
BV16-13, monoclonal
reaktywność gatunkowa (przewidywana na podstawie homologii)
all
producent / nazwa handlowa
Chemicon®
metody
ELISA: suitable
immunocytochemistry: suitable
radioimmunoassay: suitable
izotyp
IgG2aκ
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
Specyficzność
Double stranded DNA (dsDNyA) (B-form) from human. Shows 95% reactivity to dsDNA and 28% reactivity to ssDNA.
BINDING ASSAY:
Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation.
ANTIGEN BINDING
PROFILE: Relative Binding To:
Natural DNA Synthetic homopolymers
dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU)
1.00 0.45 0.01 0.01 0.01 0.01 0.01 0.01
Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 mg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0).
a) Native calf thymus DNA b) Heat-denatured calf thymus DNA
BINDING ASSAY:
Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation.
ANTIGEN BINDING
PROFILE: Relative Binding To:
Natural DNA Synthetic homopolymers
dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU)
1.00 0.45 0.01 0.01 0.01 0.01 0.01 0.01
Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 mg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0).
a) Native calf thymus DNA b) Heat-denatured calf thymus DNA
Immunogen
Epitope: double stranded, 28% reactive with single stranded
Zastosowanie
Detect DNA with Anti-DNA Antibody, double stranded, clone BV16-13 (Mouse Monoclonal Antibody), that has been shown to work in ELISA, RIA, ICC.
EIA/RIA
Immunocytochemistry
Optimal working dilutions must be determined by end user.
Immunocytochemistry
Optimal working dilutions must be determined by end user.
Research Category
Epigenetics & Nuclear Function
Apoptosis & Cancer
Epigenetics & Nuclear Function
Apoptosis & Cancer
Research Sub Category
Cell Cycle, DNA Replication & Repair
Apoptosis - Additional
Cell Cycle, DNA Replication & Repair
Apoptosis - Additional
Postać fizyczna
Unpurified tissue culture supernatant from a perfusion system, filtered through a 0.2μ micron membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL.
Przechowywanie i stabilność
Maintain at 2-8°C in undiluted aliquots for up to 6 months.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Certyfikaty analizy (CoA)
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