MAB4120
Anti-MDR1 Antibody, clone JSB-1
culture supernatant, clone JSB-1, Chemicon®
Synonim(y):
P-glycoprotein, CD243, p170, Pgp
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
culture supernatant
rodzaj przeciwciała
primary antibodies
klon
JSB-1, monoclonal
reaktywność gatunkowa
human, hamster
producent / nazwa handlowa
Chemicon®
metody
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
izotyp
IgG1
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
informacje o genach
human ... ABCB1(5243)
Specyficzność
Reacts with a conserved cytoplasmic epitope of the plasma membrane-associated 170 kDa P-gp, member of the superfamily of transmembrane transporters. JSB-1 detects P-gp overexpression in human tumor cells of all different histogenetic derivations.
JSB-1 does not cross-react with MDR3 P-glycoprotein. JSB-1 has been shown to cross-react with Pyruvate Carboxylase (PC), an abundant Mr 130,000 mitochondrial enzyme, on both immunoblots and immunohistochemical tissue sections [Rao et al. (1995). J Histo. Cytochem. 43(12):1187-1192.] Unequivocal plasma membrane patterns of immunostaining represent true P-glycoprotein ex-presssion. Weak homogeneous, cytoplasmic, or granular patterns of reactivity may represent staining of the PC cross-reactive epitope rather than positive staining for P-glycoprotein.
JSB-1 does not cross-react with MDR3 P-glycoprotein. JSB-1 has been shown to cross-react with Pyruvate Carboxylase (PC), an abundant Mr 130,000 mitochondrial enzyme, on both immunoblots and immunohistochemical tissue sections [Rao et al. (1995). J Histo. Cytochem. 43(12):1187-1192.] Unequivocal plasma membrane patterns of immunostaining represent true P-glycoprotein ex-presssion. Weak homogeneous, cytoplasmic, or granular patterns of reactivity may represent staining of the PC cross-reactive epitope rather than positive staining for P-glycoprotein.
Immunogen
Epitope: Cytoplasmic
Zastosowanie
Anti-MDR1 Antibody, clone JSB-1 detects level of MDR1 & has been published & validated for use in FC, IC, IH(P) & WB.
Flow Cytometry
Immunohistochemistry on frozen and paraffin embedded tissue
sections: 1:20.
Immunocytochemistry: acetone or air-dried preparations react well.
Western blot
Note: For cellular detection, permeabilization is required.
Optimal working dilutions must be determined by end user.
Immunohistochemistry on frozen and paraffin embedded tissue
sections: 1:20.
Immunocytochemistry: acetone or air-dried preparations react well.
Western blot
Note: For cellular detection, permeabilization is required.
Optimal working dilutions must be determined by end user.
Research Category
Metabolism
Metabolism
Research Sub Category
Toxicology & Drug Resistance
Toxicology & Drug Resistance
Opis wartości docelowych
170 kDa
Postać fizyczna
UnPurified mouse culture supernatant containing 0.7% BSA and 0.1% sodium azide as a preservative.
Unpurified
Przechowywanie i stabilność
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Komentarz do analizy
Control
MDR cells
MDR cells
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This page may contain text that has been machine translated.
Nie możesz znaleźć właściwego produktu?
Wypróbuj nasz Narzędzie selektora produktów.
polecane
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Multidrug resistance P-glycoprotein monoclonal antibody JSB-1 crossreacts with pyruvate carboxylase
Rao, V V, et al
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 43, 1187-1192 (1995)
T Yanagisawa et al.
British journal of cancer, 80(8), 1190-1196 (1999-06-22)
Clinical studies have suggested that both MDR1 and MRP may play a significant role in the chemosensitivity and outcome of neuroblastoma. To clarify the nature of multidrug resistance (MDR) in this tumour a series of six neuroblastoma cell lines have
Daria Brambilla et al.
International journal of cancer, 130(12), 2824-2834 (2011-07-23)
Overexpression of the mdr1 gene encoding P-glycoprotein (Pgp) exerts a major role in reducing the effectiveness of cytotoxic therapy in osteosarcoma. The interaction between actin and Pgp has been shown to be instrumental in the establishment of multidrug resistance (MDR)
Andrea Ries Thurman et al.
Journal of acquired immune deficiency syndromes (1999), 78(1), 82-92 (2018-02-10)
We describe and compare the local and systemic pharmacokinetics (PK) of tenofovir (TFV) and TFV-diphosphate (TFV-DP) in healthy premenopausal (PRE) and postmenopausal (POST) women using TFV 1% gel and correlate local PK with other mucosal end points. PRE (n =
Matthew W Wilson et al.
Investigative ophthalmology & visual science, 47(4), 1269-1273 (2006-03-28)
To compare the expression of multidrug-resistant proteins in retinoblastoma tumors among eyes treated with primary enucleation. A group of 18 patients with unilateral retinoblastoma with advanced intraocular disease was selected for the study. All patients had undergone primary enucleation. A
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej