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Merck

AP1064

PhosphoDetect Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb

liquid, Calbiochem®

Synonim(y):

Anti-Pyruvate Dehydogenase pSer³⁰⁰ Rabbit pAb

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Wybierz wielkość

50 μG

1680,00 zł

1 MG

11 250,00 zł

1680,00 zł

Dostępne do wysyłki DZISIAJSzczegóły

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Informacje o tej pozycji

NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
human
Application:
—
Citations:
32

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biological source

rabbit

Quality Level

200

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer300)

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Ta pozycja
AP1063DR1025506119
PhosphoDetect Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb liquid, Calbiochem®

Sigma-Aldrich

AP1064

PhosphoDetect Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb

PhosphoDetect Anti-PDH-E1α (pSer²³²) Rabbit pAb liquid, Calbiochem®

Sigma-Aldrich

AP1063

PhosphoDetect Anti-PDH-E1α (pSer²³²) Rabbit pAb

PhosphoDetect Anti-Chk1 (pSer³¹⁷) Rabbit pAb liquid, Calbiochem®

Sigma-Aldrich

DR1025

PhosphoDetect Anti-Chk1 (pSer³¹⁷) Rabbit pAb

PhosphoDetect Anti-p38 MAP Kinase (pThr¹⁸⁰, pTyr¹⁸²) Rabbit pAb liquid, Calbiochem®

Sigma-Aldrich

506119

PhosphoDetect Anti-p38 MAP Kinase (pThr¹⁸⁰, pTyr¹⁸²) Rabbit pAb

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

form

liquid

form

liquid

form

liquid

form

liquid

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser300.
Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser300 in HEK293 cells.
This PhosphoDetect Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry for the detection of PDH-E1α (pSer³⁰⁰).

Immunogen

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁰⁰ phosphorylation site of human PDH-E1α

Application



Immunoblotting (0.5 μg/ml)
Immunocytochemistry (1 μg/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
HEK293 cells treated with dichloroacetate
Positive Control
HEK293 cells

Other Notes

Due to a high degree of sequence identity, this antibody is also expected to cross-react with mouse, rat, bovine, Zebra fish, and Xenopus phospho-PDH-E1α Antibody should be titrated for optimal results in individual systems.
Rardin M.J., et. al. 2009. Anal. Biochem.2, 157.
Seifert, F., et al. 2007. Biochemistry 21, 6277.
Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans.34, 217.
Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)
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Klasa składowania

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Yu Juan Chai et al.
Plants (Basel, Switzerland), 10(9) (2021-09-29)
Three undescribed bicyclo[3.2.1]octanoid neolignan glucosides, along with a known neolignan, were isolated from the leaves of Piper crocatum Ruiz & Pav. Their chemical structures were elucidated using extensive spectroscopic analyses including 1D and 2D NMR experiments and HR-ESI-MS analysis, as
David Morales-Alamo et al.
Frontiers in physiology, 9, 188-188 (2018-04-05)
Compared to normoxia, during sprint exercise in severe acute hypoxia the glycolytic rate is increased leading to greater lactate accumulation, acidification, and oxidative stress. To determine the role played by pyruvate dehydrogenase (PDH) activation and reactive nitrogen and oxygen species
Gretchen L Seim et al.
Nature metabolism, 1(7), 731-742 (2020-04-08)
In response to signals associated with infection or tissue damage, macrophages undergo a series of dynamic phenotypic changes. Here we show that during the response to LPS and interferon-γ stimulation, metabolic reprogramming in macrophages is also highly dynamic. Specifically, the
Changyeol Lee et al.
Molecules (Basel, Switzerland), 24(24) (2019-12-11)
The fungal strain Alternaria alternata JS0515 was isolated from Vitex rotundifolia (beach vitex). Twelve secondary metabolites, including one new altenusin derivative (1), were isolated. The isolated metabolites included seven known altenusin derivatives (2-8), two isochromanones (9, 10), one perylenequinone (11)
Lifeng Yuan et al.
Cell research, 28(6), 625-643 (2018-05-29)
Cellular senescence is a fundamental cell fate playing a significant role throughout the natural aging process. However, the molecular determinants distinguishing senescence from other cell-cycle arrest states such as quiescence and post-mitotic state, and the specified mechanisms underlying cell-fate decisions
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SKUNUMER GTIN
AP1064-1MG04055977227796
AP1064-50UG04055977227802

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