ABE44

Anti-trimethyl Histone H3 (Lys27) Antibody

Name: Anti-trimethyl Histone H3 (Lys27) Antibody
Brand: MM
Price: 2310 PLN

from rabbit, purified by affinity chromatography

Synonim(y):

H3K27me3, Histone H3 (tri methyl K27), H3 histone family, member T, histone 3, H3, histone cluster 3, H3, H3K27me3

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Wybierz wielkość

100 μG

2310,00 zł

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Informacje o tej pozycji

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Clone:

polyclonal

Species reactivity:

mouse, human

Application:

DB, ICC, WB, inhibition assay

Citations:

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biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

technique(s)

dot blot: suitable, immunocytochemistry: suitable, inhibition assay: suitable (peptide), western blot: suitable

NCBI accession no.

NP_003484.1

UniProt accession no.

Q16695

shipped in

wet ice

target post-translational modification

trimethylation (Lys27)

Gene Information

human ... HIST1H3F(8968)

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Ta pozycja	ABE244	ABE328	ABE375
Sigma-Aldrich ABE44 Anti-trimethyl Histone H3 (Lys27) Antibody	Sigma-Aldrich ABE244 Anti-monomethyl-Histone H3 Antibody (Lys36)	Sigma-Aldrich ABE328 Anti-dimethyl Histone H3 (Arg2) Antibody	Sigma-Aldrich ABE375 Anti-acetyl-phospho Histone H1.4/H3 (Lys26/Lys9, Ser27/Ser10) Antibody
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
technique(s) dot blot: suitable, inhibition assay: suitable (peptide), immunocytochemistry: suitable, western blot: suitable	technique(s) dot blot: suitable, immunoprecipitation (IP): suitable, western blot: suitable	technique(s) dot blot: suitable, western blot: suitable	technique(s) dot blot: suitable, immunoprecipitation (IP): suitable, western blot: suitable
UniProt accession no. Q16695	UniProt accession no. Q16695	UniProt accession no. P68431	UniProt accession no. P68431
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal

General description

Histone H3 is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3 lysine 27 methylation (H3K27) is associated with heterochromatin formation and transcriptional repression.

~ 17 kDa

Immunogen

Branch peptide corresponding to human Histone H3 methylated at Lys27.

Epitope: Lys27

Application

Anti-trimethyl Histone H3 (Lys27) Antibody is a rabbit polyclonal antibody for detection of Histone H3 trimethylated on lysine 27( H3K27me3). This purified polyclonal is specificity verified by dot blot (DB), published in peer reviewed journals and validated in WB, ICC, PIA.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone H3 in NIH/3T3 cells.
Peptide Inhibition Assay: This antibody peptide blocked on HeLa cell extracts.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Biochem/physiol Actions

Broad species cross-reactivity expected.

This antibody recognizes Histone H3 methylated at Lys27.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa acid extract and recombinant Histone H3

Evaluated by Western Blot in HeLa acid extract and recombinant Histone H3.

Western Blot Analysis: 0.1 µg/ml of this antibody detected histone H3 in 10 µg of HeLa acid extract .

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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12 - Non Combustible Liquids

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WGK 1

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Chromatin signature of widespread monoallelic expression.

Nag, A; Savova, V; Fung, HL; Miron, A; Yuan, GC; Zhang, K; Gimelbrant, AA

eLife null

Chromatin Signature Identifies Monoallelic Gene Expression Across Mammalian Cell Types.

Nag, A; Vigneau, S; Savova, V; Zwemer, LM; Gimelbrant, AA

G3 (Bethesda, Md.) null

DDX3 Represses Stemness by Epigenetically Modulating Tumor-suppressive miRNAs in Hepatocellular Carcinoma.

Hao-Kang Li et al.

Scientific reports, 6, 28637-28637 (2016-06-28)

Studies indicate that the presence of cancer stem cells (CSCs) is responsible for poor prognosis of hepatocellular carcinoma (HCC) patients. In this study, the functional role of DDX3 in regulation of hepatic CSCs was investigated. Our results demonstrated that reduced

Polycomb- and Methylation-Independent Roles of EZH2 as a Transcription Activator.

Jung Kim et al.

Cell reports, 25(10), 2808-2820 (2018-12-06)

Enhancer of Zeste 2 (EZH2) is the enzymatic subunit of Polycomb Repressive Complex 2 (PRC2), which catalyzes histone H3 lysine 27 trimethylation (H3K27me3) at target promoters for gene silencing. Here, we report that EZH2 activates androgen receptor (AR) gene transcription

Development of multiple cell-based assays for the detection of histone H3 Lys27 trimethylation (H3K27me3).

Qian, J; Lu, L; Wu, J; Ma, H

Assay and Drug Development Technologies null

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SKU	NUMER GTIN
ABE44	04053252675270

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