AB10518

Anti-DARPP-32 Antibody

from rabbit, purified by affinity chromatography

• Synonim(y): protein phosphatase 1, regulatory (inhibitor) subunit 1B, dopamine and cAMP-regulated neuronal phosphoprotein 32, dopamine and cAMP regulated phosphoprotein, protein phosphatase 1 regulatory subunit 1B, Dopamine- and cAMP-regulated neuronal phosphoprotei
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IP, WB
• Citations: 22

Właściwości

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat, mouse, rhesus macaque
• species reactivity (predicted by homology): canine (based on 100% sequence homology), rhesus monkey (based on 100% sequence homology)
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• NCBI accession no.: NP_659077
• UniProt accession no.: Q60829
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Ppp1R1B(19049)

Opis

General description

Dopamine- and cAMP-regulated neuronal phosphoprotein (DARP-32) is a cytoplasmic member of the protein phosphatase inhibitor 1 family. DARP-32 is a multifunctional protein kinase and protein phosphatase regulator, depending on the site of its activation. It possesses at least four sites that can be phosphorylated. Phosphorylation by PKA at Thr34 stimulates DARP-32 to act as a protein-phosphatase-1 (PP-1) inhibitor. However, phosphorylation at Thr75 will inhibit Thr34 phosphorylation. This selective, bidirectional regulation allows DARPP-32 to have a wide range of influence on downstream physiological effectors such as: ion pumps, neurotransmitter receptors, voltage-gate ion channels, and transcriptional factors. Studies suggest that it may also have a role in the Circadian System photic pathway. Overexpression of DARPP-32 has been linked with antiapoptotic mechanisms of cancer cells and has been observed in breast, colon, esophagus, gastric, and prostate cancer tissues.

~ 32 kDa observed

Immunogen

KLH-conjugated linear peptide corresponding to mouse DARPP-32 near the N-terminus.

Application

Anti-DARPP-32 Antibody is an antibody against DARPP-32 for use in WB & IP.

Research Sub Category
Neurotransmitters & Receptors

Ion Channels & Transporters

Western Blot (Snap i.d.) Analysis: 1 µg/mL of this antibody from a previous lot detected DARPP-32 on 10 µg of rat brain tissue lysate.

Immunoprecipitaion Analysis: 10 µg of this antibody from a previous lot immunoprecipitated DARPP-32 from rat brain RIPA lysate.

Biochem/physiol Actions

This antibody recognizes DARPP-32 near the N-terminus.

Analysis Note

Evaluated by Western Blot on rat brain tissue lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected DARPP-32 on 10 µg of rat brain tissue lysate.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Replaces: AB1656

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable