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BugBuster® 10X Protein Extraction Reagent

BugBuster 10X is a concentrated formulation of the protein extraction reagent, using proprietary detergents employed in BugBuster without the addition of salts or buffer components.

Synonim(y):

BugBuster Reagent

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Gabaryty przesyłkiSKUDostępnośćCena netto
10 mL

Dostępne do wysyłki DZISIAJzKuehne + Nagel Sp. z o.o.

503,00 zł
50 mL

Przewidywany termin wysyłki07 kwietnia 2026zKuehne + Nagel Sp. z o.o.

2330,00 zł
100 mL

Dostępne do wysyłki DZISIAJzKuehne + Nagel Sp. z o.o.

5410,00 zł

Informacje o tej pozycji

NACRES:
NA.77
UNSPSC Code:
41106511

503,00 zł


Dostępne do wysyłki DZISIAJSzczegóły


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Quality Level

form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

technique(s)

protein purification: suitable

suitability

suitable for column chromatography

application(s)

life science and biopharma

shipped in

ambient

storage temp.

10-30°C

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Ta pozycja
7092270584-M70923
form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

protein purification: suitable

technique(s)

-

technique(s)

-

technique(s)

-

suitability

suitable for column chromatography

suitability

-

suitability

-

suitability

-

application(s)

life science and biopharma

application(s)

-

application(s)

-

application(s)

-

storage temp.

10-30°C

storage temp.

2-8°C

storage temp.

10-30°C

storage temp.

10-30°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

General description

BugBuster 10X is a concentrated formulation of the proprietary detergents employed in BugBuster without the addition of salts or buffer components. Concentrated BugBuster provides a flexible alternative to the ready-to-use standard 1X BugBuster, allowing user-defined dilution and addition of buffer components. BugBuster 10X has all of the bioprocessing benefits of standard BugBuster plus the freedom to control pH, reagent concentration, and buffer additives necessary for maximum extraction and activity of your target protein.

Features and Benefits

Concentrated formulation: BugBuster 10X is a highly concentrated protein extraction reagent that offers a flexible alternative to the ready-to-use standard 1X BugBuster. This means that users can define their dilution and add buffer components to extract the maximum amount of target protein from the sample.

Proprietary detergents: The proprietary detergents used in BugBuster 10X provide all the benefits of the standard BugBuster reagent, such as efficient lysis of bacterial and yeast cells. The absence of salts and buffer components ensures that the extracted protein is of high purity and free from contaminants.

User-defined control: With BugBuster 10X, users have complete control over the pH, reagent concentration, and buffer additives necessary for optimal protein extraction and activity. This level of customization makes it an ideal solution for working on protein research with unique requirements.

Pain points addressed: BugBuster 10X addresses the pain points including but not limited to: low yields, inefficient extraction, and protein degradation. Its concentrated formulation and user-defined control offer a flexible and efficient solution to protein extraction, resulting in high-quality protein samples for downstream applications.

Legal Information

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)
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Klasa składowania

12 - Non Combustible Liquids


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Powiązane treści

Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.

Traditionally, protein purification from E. coli consists of four distinct phases: harvest, bacterial cell lysis, lysate clarification and protein purification. Bacterial lysis typically requires several time-consuming, hands-on steps, such as freeze/thaw cycles and sonication. These harsh lysis techniques may negatively impact protein quality and contribute to sample-to-sample variability. To maintain protein activity and integrity, detergent-based lysis buffers are routinely used to avoid mechanical protein extraction methods. Regardless of the lysis method used, centrifugation is traditionally required to pellet unwanted cell debris and permit recovery of the clarified lysate. The final step, purification, is frequently performed using affinity media specific for expressed epitope tags. Agarose-based media have typically been used, either as a slurry in microcentrifuge tubes or packed into gravity-driven or spin columns. While easier to manipulate, columns are greatly affected by lysate consistency and carryover of cell debris, which can lead to clogging of the column frits.

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Numer pozycji handlu globalnego

SKUNUMER GTIN
70921-504055977272741
70921-304055977272727
70921-404055977272734

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