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70584-M

Millipore

BugBuster® Protein Extraction Reagent

Synonim(y):

Protein Extraction Reagent, Protein Extraction Solution

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About This Item

Kod UNSPSC:
41106516
NACRES:
NA.32

Formularz

liquid

Poziom jakości

100

producent / nazwa handlowa

Novagen®

warunki przechowywania

OK to freeze
avoid repeated freeze/thaw cycles

temp. przechowywania

10-30°C

Opis ogólny

BugBuster® Protein Extraction Reagent is formulated for gentle disruption of Escherichia coli cell wall to liberate active proteins. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The proprietary formulation utilizes a tris-buffer-based mixture of non-ionic and zwitterionic detergents that is capable of cell wall perforation without denaturing the protein. BugBuster is also useful for the preparation of high-purity inclusion bodies for subsequent purification in instances where expressed proteins are insoluble.

Zastosowanie

BugBuster® Protein Extraction Reagent has been used:
  • to lyse Escherichia coli BLR (DE3) cells for β-glucosidase (BglB) production and purification
  • as a cell lysis buffer to treat the synechocystis PCC6803 transconjugants and measure the yellow fluorescent protein (YFP) fluorescence
  • in lysis buffer for purification of glutathione S-transferase- heat shock protein family A (Hsp70) member 8 (GST- HSPA8)

Ostrzeżenie

Toxicity: Standard Handling (A)

Informacje prawne

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Ta strona może zawierać tekst przetłumaczony maszynowo.

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Patricia Armshaw et al.
Biotechnology for biofuels, 8, 201-201 (2015-12-03)
The use of photosynthetic autotrophs and in particular the model organism Synechocystis PCC6803 is receiving much attention for the production of sustainable biofuels and other economically useful products through metabolic engineering. Optimisation of metabolic-engineered organisms for high-level sustained production of
Dylan Alexander Carlin et al.
PloS one, 12(5), e0176255-e0176255 (2017-05-23)
Accurate modeling of enzyme activity and stability is an important goal of the protein engineering community. However, studies seeking to evaluate current progress are limited by small data sets of quantitative kinetic constants and thermal stability measurements. Here, we report
Tiejian Nie et al.
Autophagy, 17(10), 2923-2938 (2021-05-11)
As a highly dynamic organelle, mitochondria undergo constant fission and fusion to change their morphology and function, coping with various stress conditions. Loss of the balance between fission and fusion leads to impaired mitochondria function, which plays a critical role

Produkty

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

BugBuster® and Benzonase® Reagents are the Clear Solutions to Simple, Efficient Extraction of E. coli Proteins

This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.

ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

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Lysis & Protein Extraction

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