393206
Anti-HNE Fluorophore Rabbit pAb
liquid, Calbiochem®
Synonim(y):
Anti-N α-Acetyllysine-4-hydroxy-2-nonenal Fluorophore, Anti-Nα-Acetyllysine-4-hydroxy-2-nonenal Fluorophore
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About This Item
Kod UNSPSC:
12352203
NACRES:
NA.43
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
diluted serum
rodzaj przeciwciała
primary antibodies
klon
polyclonal
Postać
liquid
reaktywność gatunkowa (przewidywana na podstawie homologii)
all
producent / nazwa handlowa
Calbiochem®
warunki przechowywania
OK to freeze
avoid repeated freeze/thaw cycles
izotyp
IgG
Warunki transportu
wet ice
temp. przechowywania
−70°C
docelowa modyfikacja potranslacyjna
unmodified
Opis ogólny
Anti-HNE Fluorophore, rabbit polyclonal, recognizes the HNE-derived chromphohore of the 2:1 lysine-HNE-fluorophore. It is validated for use in ELISA, Western blotting, and immunohistochemistry.
Rabbit polyclonal antibody supplied as diluted serum. Recognizes the HNE-derived chromophore of the 2:1 lysine-HNE fluorophore.
Recognizes the HNE-derived chromphohore of the 2:1 lysine-HNE-fluorophore.
Immunogen
a 2:1 Nα-acetyllysine-HNE fluorophore, conjugated to KLH
Zastosowanie
ELISA (1:500-1:1000)
Immunoblotting (1:1000-1:5000)
Immunohistochemistry (1:100-1:500)
Immunoblotting (1:1000-1:5000)
Immunohistochemistry (1:100-1:500)
Ostrzeżenie
Toxicity: Standard Handling (A)
Postać fizyczna
Serum diluted 1:1 with glycerol.
Rekonstytucja
Following initial thaw, aliquot and freeze (-70°C).
Inne uwagi
Due to the small epitope size, overnight incubation with the antibody greatly increases sensitivity by immunoblotting. Determined by competitive ELISA and immunoblotting to recognize the chromophore structure of fluorophore derived from the reaction of 4-HNE with primary amines. Does not recognize 1:1 cysteine-, 1:1 histidine-, or 1:1 lysine-HNE Michael Adducts. Variables associated with assay conditions will dictate the proper working dilution.
Tsai, L., et al. 1998. Proc. Natl. Acad. Sci. USA95, 7975.
Informacje prawne
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
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