16-318

Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Magnetic Bead Conjugate

clone SKB1, from mouse

• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: SKB1, monoclonal
• reaktywność gatunkowa: mouse, rat, human
• metody: immunoprecipitation (IP): suitable
• izotyp: IgG
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: mouse ... Akt1(11651)

Opis

Opis ogólny

Akt (protein kinase B), a serine/threonine kinase, has emerged as a critical enzyme in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. In mammals three isoforms of Akt (α, β, γ or Akt 1, 2, 3) are reported that exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt α is the predominant isoform in most tissues, whereas the highest expression of Akt β is observed in the insulin-responsive tissues, and Akt γ is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.

Specyficzność

Recognizes Akt1/PKBα.

Immunogen

GST-fusion protein corresponding to residues 1-149 of human Akt1

Zastosowanie

Detect Akt/PKB using this Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Magnetic Bead Conjugate validated for use in IP.

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

Jakość

Evaluated by immunoprecipitation on 250 μg of NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes).

Opis wartości docelowych

~ 60 kDa observed

Postać fizyczna

20% slurry; 1X PBS, 0.05% sodium azide.
Sufficient for 10 IPs.

Format: Purified

Protein G Chromatography

Przechowywanie i stabilność

Stable for 1 year at 2-8ºC from date of receipt.

Komentarz do analizy

Control
NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 2