1.08488

Urea

for biochemistry

• Synonim(y): Carbamide, Carbonyl diamide, Diaminomethanone, Carbonyldiamine, Carbamide, Carbonyldiamide
• Wzór liniowy: NH₂CONH₂
• Numer CAS: 57-13-6
• Masa cząsteczkowa: 60.06
• UNSPSC Code: 12352111
• NACRES: NA.21
• EC Index Number: 200-315-5
• MDL number: MFCD00008022
• Beilstein/REAXYS Number: 635724

Właściwości

• vapor pressure: <0.1 hPa ( 20 °C)
• Quality Level: 100
• form: solid
• potency: 8471 mg/kg LD₅₀, oral (Rat), 8200 mg/kg LD₅₀, skin (Rat)
• technique(s): titration: suitable
• pH: 9 (20 °C, 100 g/L in H₂O)
• mp: 132-135 °C (lit.)
• solubility: 1000 g/L
• density: 1.335 g/mL at 25 °C (lit.)
• bulk density: 720‑760 kg/m³
• storage temp.: 10-30°C
• SMILES string: NC(N)=O
• InChI: 1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
• InChI key: XSQUKJJJFZCRTK-UHFFFAOYSA-N

Opis

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Analysis Note

Purity (DSC (differential scanning calorimetry)): ≥ 99.5 %
Identity (IR-spectrum): passes test
Appearance: Colorless crystals or white crystalline powder.
Appearance of solution (100 g/l, water): Clear and colorless.
Melting point (DSC (differential scanning calorimetry)): 132 - 135 °C
UV-Absorption (260 nm; 8 mol/l; 1 cm; water): ≤ 0.1
UV-Absorption (280 nm; 8 mol/l; 1 cm; water): ≤ 0.1
Cyanide (CN): ≤ 0.000001 %
Chloride (Cl): ≤ 0.0005 %
Sulfate (SO₄): ≤ 0.001 %
Heavy metals (as Pb): ≤ 0.0004 %
Fe (Iron): ≤ 0.00002 %
NH₄ (Ammonium): ≤ 0.00001 %
Biuret: ≤ 0.05 %
Sulfated ash (800 °C): ≤ 0.01 %

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable