07-452
Anti-dimethyl-Histone H3 (Lys27) Antibody
Upstate®, from rabbit
Synonim(y):
H3K27me2, Histone H3 (di methyl K27)
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
affinity purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
polyclonal
oczyszczone przez
affinity chromatography
reaktywność gatunkowa
human
producent / nazwa handlowa
Upstate®
metody
ChIP: suitable (ChIP-seq)
cell based assay: suitable
dot blot: suitable
multiplexing: suitable
western blot: suitable
izotyp
IgG
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
dimethylation (Lys27)
informacje o genach
human ... H3C1(8350)
Opis ogólny
Anti-dimethyl-Histone H3 (Lys27)
Specyficzność
Broad species cross-reactivity expected, based on sequence homology
Recognizes dimethylated histone H3 (Lys27).
Immunogen
KLH-conjugated, synthetic 2X-branched peptide containing the sequence ...ARme2KSA... in which me2K corresponds to dimethyl-lysine at residue 27 of human histone H3
Zastosowanie
Additional Referenced Research Applications:
Dot Blot, Immunocytochemistry (cells)
Dot Blot, Immunocytochemistry (cells)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-dimethyl-Histone H3 (Lys27) Antibody (rabbit polyclonal antibody) validated in Mplex, PIA, WB, ChIP-seq, DB to detect dimethyl-Histone H3 (Lys27) also known as H3K27me2, Histone H3 (di methyl K27).
Jakość
Routinely evaluated by immunoblot on acid extracted proteins from HeLa cells.
Opis wartości docelowych
17 kDa
Postać fizyczna
Affinity purified IgG in 0.1M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% NaN3.
Peptide affinity purified
Przechowywanie i stabilność
Stable for 2 years at 2-8°C from date of shipment.
Komentarz do analizy
Control
HeLa whole cell lysate, U2OS cell lysate
HeLa whole cell lysate, U2OS cell lysate
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Epigenetic regulation of dendritic cell-derived interleukin-12 facilitates immunosuppression after a severe innate immune response.
Wen, H; Dou, Y; Hogaboam, CM; Kunkel, SL
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Establishment of histone h3 methylation on the inactive X chromosome requires transient recruitment of Eed-Enx1 polycomb group complexes.
Silva, Jose, et al.
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Reciprocal patterns of methylation of H3K36 and H3K27 on proximal vs. distal IgVH genes are modulated by IL-7 and Pax5.
Xu, CR; Schaffer, L; Head, SR; Feeney, AJ
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Pedro Crevillén et al.
Nature, 515(7528), 587-590 (2014-09-16)
The reprogramming of epigenetic states in gametes and embryos is essential for correct development in plants and mammals. In plants, the germ line arises from somatic tissues of the flower, necessitating the erasure of chromatin modifications that have accumulated at
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