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QR0200

Quantitative RT-PCR ReadyMix™

One step RT-qPCR for probe-based methods, MMLV & hot-start Taq

Synonim(y):

1-step RT-qPCR mix, Quantitative real-time PCR master mix

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Gabaryty przesyłkiSKUDostępnośćCena netto
1 kit
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
2360,00 zł

Informacje o tej pozycji

NACRES:
NA.55
UNSPSC Code:
41106300

2360,00 zł

Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
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Quality Level

300

usage

sufficient for 250 reactions

feature

dNTPs included, hotstart

technique(s)

RT-qPCR: suitable, qPCR: suitable

color

colorless

input

purified RNA

compatibility

ABI 5700, ABI 7000, ABI 7300, ABI 7500 Fast, ABI 7500, ABI 7700, ABI 7900 HT , ABI 7900 HT Fast, ABI 7900, ABI StepOne, ABI StepOnePlus, ABI ViiA 7, Bio-Rad CFX384, Bio-Rad CFX96, Bio-Rad MJ Chromo4, Bio-Rad MJ Opticon 2, Bio-Rad MJ Opticon, Bio-Rad MiniOpticon, Bio-Rad MyiQ, Bio-Rad iCycler iQ, Bio-Rad iQ5, Cepheid SmartCycler, Eppendorf® Mastercycler ep realplex2 s, Eppendorf® Mastercycler ep realplex, Illumina Eco qPCR, Qiagen Corbett Rotor-Gene 3000, Qiagen Corbett Rotor-Gene 6000, Qiagen Corbett Rotor-Gene Q, Roche LightCycler 480, Strategene Mx3000P, Strategene Mx3005P, Strategene Mx4000

detection method

probe-based

shipped in

wet ice

storage temp.

−20°C

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Ta pozycja
S9194P4600D7440
Quantitative RT-PCR ReadyMix™ One step RT-qPCR for probe-based methods, MMLV & hot-start Taq

Sigma-Aldrich

QR0200

Quantitative RT-PCR ReadyMix™

SYBR® Green JumpStart™ Taq ReadyMix™ for High Throughput qPCR SYBR® Green qPCR reagent, passive reference dye included

Sigma-Aldrich

S9194

SYBR® Green JumpStart™ Taq ReadyMix™ for High Throughput qPCR

ReadyMix™ Taq PCR Reaction Mix with MgCl2

Sigma-Aldrich

P4600

ReadyMix™ Taq PCR Reaction Mix

JumpStart™ Taq ReadyMix™ for Quantitative PCR For probe-based real-time PCR

Sigma-Aldrich

D7440

JumpStart™ Taq ReadyMix™ for Quantitative PCR

technique(s)

RT-qPCR: suitable

technique(s)

qPCR: suitable

technique(s)

PCR: suitable

technique(s)

RT-qPCR: suitable

detection method

probe-based

detection method

SYBR® Green

detection method

-

detection method

probe-based

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

usage

sufficient for 250 reactions

usage

sufficient for 20 reactions, sufficient for 2000 reactions, sufficient for 400 reactions

usage

sufficient for 100 reactions

usage

sufficient for 100 reactions, sufficient for 20 reactions, sufficient for 400 reactions

compatibility

ABI 5700, ABI 7300, ABI 7500, ABI 7900 HT , ABI StepOnePlus, Bio-Rad CFX384, Bio-Rad MJ Chromo4, Bio-Rad MJ Opticon, Bio-Rad MyiQ, Bio-Rad iQ5, Eppendorf® Mastercycler ep realplex2 s, Illumina Eco qPCR, Qiagen Corbett Rotor-Gene 6000, Roche LightCycler 480, Strategene Mx3005P, ABI 7000, ABI 7700, Bio-Rad CFX96, Bio-Rad MiniOpticon, Cepheid SmartCycler, Qiagen Corbett Rotor-Gene 3000, Strategene Mx3000P, ABI 7500 Fast, ABI ViiA 7, Bio-Rad iCycler iQ, Qiagen Corbett Rotor-Gene Q, ABI 7900 HT Fast, Bio-Rad MJ Opticon 2, Strategene Mx4000

compatibility

for use with ABI 5700, for use with ABI 7000, for use with ABI 7300, for use with ABI 7700, for use with ABI 7900 Fast, for use with ABI 7900 HT, for use with ABI StepOne, for use with ABI StepOnePlus

compatibility

-

compatibility

Bio-Rad MyiQ ( ), Bio-Rad iCycler iQ, Bio-Rad iQ 5, for use with ABI 5700, for use with ABI 7000, for use with ABI 7300, for use with ABI 7500 Fast, for use with ABI 7700, for use with ABI 7900 Fast, for use with ABI 7900 HT, for use with ABI StepOne, for use with ABI StepOnePlus, for use with ABI ViiA 7, for use with Bio-Rad CFX384, for use with Bio-Rad CFX96, for use with Bio-Rad MJ Chromo4, for use with Bio-Rad MJ Opticon 2, for use with Bio-Rad MJ Opticon Cepheid SmartCycler, for use with Bio-Rad MiniOpticon, for use with Eppendorf® Mastercycler ep realplex2 s, for use with Illumina Eco qPCR, for use with Qiagen Corbett Rotor-Gene 3000, for use with Qiagen Corbett Rotor-Gene 6000, for use with Qiagen Corbett Rotor-Gene Q, for use with Roche LightCycler 480, for use with Strategene Mx3000P, for use with Strategene Mx3005P, for use with Strategene Mx4000

General description

Quantitative RT-PCR ReadyMix™ combines Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and JumpStart™ Taq DNA polymerase in a one-step RT-PCR kit designed for the measurement of gene expression. This convenient 2X master mix includes M-MLV RT, JumpStart™ Taq DNA polymerase, 99% pure deoxynucleotides, buffer, glass passivator, and stabilizers. The JumpStart™ Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. This kit has been formulated for fluorogenic hybridization probe-based detection methods.

Application

Quantitative RT-PCR ReadyMix™ has been used in the quantification of messenger RNA (mRNA) levels of specific expressed genes by quantitative reverse-transcription polymerase chain reaction (RT-qPCR).[1]

Features and Benefits

  • The master mix allows consistency and reproducibility from one reaction to the next
  • Reduced risk of contamination from multiple pipetting steps
  • Reduced set-up time as compared to manual or wax Hot Start methods
  • JumpStart™Taq Polymerase reduces primer-dimer and non-specific product formation
  • Broad instrument compatibility
  • Includes a separate ROX reference dye vial for reaction normalization

Packaging

1 kit sufficient for 250 reactions at 20 μL each or for 100 reactions at 50 μL each.

Legal Information

This product is for research use only.
Eppendorf is a registered trademark of Eppendorf SE
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Ta strona może zawierać tekst przetłumaczony maszynowo.

Tylko elementy zestawu

Numer produktu
Opis
  • Probe Based qRT-PCR ReadyMix 2 X
  • Moloney Murine Leukemia Viral Reverse Transcriptase (M-MLV RT) 5000 U

Elementy zestawu są też dostępne oddzielnie

Numer produktu
Opis
Karta charakterystyki
  • P219210X PCR Buffer, Optimized for routine PCR with MgCl2 included 1.5 mL/vialKarta charakterystyki
  • M8787Magnesium chloride solution, PCR Reagent, 25 mM MgCI2 solution for PCR 25 mMKarta charakterystyki
  • P219210X PCR Buffer, Optimized for routine PCR with MgCl2 included 100 XKarta charakterystyki

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TRI Reagent®, BD, For processing whole blood, plasma, or serum.

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pictograms

Exclamation markEnvironment
GHS07,GHS09

Klasa składowania

12 - Non Combustible Liquids

wgk

WGK 3

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
SLCH0651
SLCG8790
SLCG9928
SLCG4184
SLCG4181

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Validation of Quantitative PCR Assays
Lovatt, A., et al.
BioPharm., 22-32 (2002)
A screen of shRNAs targeting tumor suppressor genes to identify factors involved in A549 paclitaxel sensitivity
Ji D, et al.
Oncology Reports, 18(6), 1499-1505 (2007)
An Integrated High-Throughput System for mRNA Purification and Quantitation for Use in Identifying Gene Knockdown by RNA Interference
Wang, et al.
JALA: Journal of the Association for Laboratory Automation, 11, 314-318 (2006)
S A Bustin
Journal of molecular endocrinology, 29(1), 23-39 (2002-08-30)
The fluorescence-based real-time reverse transcription PCR (RT-PCR) is widely used for the quantification of steady-state mRNA levels and is a critical tool for basic research, molecular medicine and biotechnology. Assays are easy to perform, capable of high throughput, and can
T B Morrison et al.
BioTechniques, 24(6), 954-958 (1998-06-19)
Continuous fluorescence observation of amplifying DNA allows rapid and accurate quantification of initial transcript copy number. A simple and generic method for monitoring product synthesis with the double-stranded DNA dye, SYBR Green I provides initial template copy number estimation limited

Produkty

Probe QPCR for Gene-Level Expression

qPCR investigates gene expression, amplification, and alterations, crucial for tumor biology and understanding cancer genetics.

Quantitative RT-PCR

RT-qPCR products combine the effective of Reverse Transcriptase with hot-start taq-directed antibody in convenient ReadyMixes for probe-based or SYBR® Green based applications.

Odwrotna transkrypcja

Wyzwania w analizie ekspresji genów obejmują stabilność mRNA, czasowe wzorce transkrypcji i korelację mRNA-białko, co wpływa na dokładność.

Ilościowy RT-PCR

Produkty RT-qPCR łączą w sobie skuteczność odwrotnej transkryptazy z przeciwciałem ukierunkowanym na gorący start taq w wygodnych mieszankach ReadyMix do zastosowań opartych na sondach lub SYBR® Green.

Zobacz wszystko

Protokoły

Reverse Transcription Protocol (One-step Probe Detection)

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

Powiązane treści

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RT-qPCR – Quantitative Reverse Transcription PCR

RT-qPCR detects specific targets with applications in gene expression and pathogen detection.

RT-qPCR - ilościowy PCR z odwrotną transkrypcją

RT-qPCR wykrywa określone cele z zastosowaniami w ekspresji genów i wykrywaniu patogenów.

Numer pozycji handlu globalnego

SKUNUMER GTIN
QR0200-1KT04061835516742

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