Przejdź do zawartości
Merck
Wszystkie zdjęcia(2)

Kluczowe dokumenty

Wyświetl całą dokumentację

U0880

Sigma-Aldrich

Uricase from Candida sp.

Uricase from Candida sp.

recombinant, expressed in E. coli, lyophilized powder, ≥2 units/mg solid

Synonim(y):

Urate: oxygen oxoreductase

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych

Wybierz wielkość

250 UNITS
657,00 zł
1000 UNITS
1950,00 zł

657,00 zł

Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Poproś o zamówienie zbiorcze
Wszystkie zdjęcia(2)

Wybierz wielkość

Zmień widok
250 UNITS
657,00 zł
1000 UNITS
1950,00 zł

About This Item

Numer CAS:
9002-12-4
Numer EC enzymu:
1.7.3.3 (BRENDA, IUBMB)
Numer MDL:
MFCD00082126
Kod UNSPSC:
12352204
Identyfikator substancji w PubChem:
24900619
NACRES:
NA.54

657,00 zł

Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Poproś o zamówienie zbiorcze

rekombinowane

expressed in E. coli

Formularz

lyophilized powder

aktywność właściwa

≥2 units/mg solid

masa cząsteczkowa

35 kDa by SDS-PAGE

temp. przechowywania

−20°C

ciąg SMILES

[P](=O)([O-])(OC3C(OC(C3O)N4C=CC(=O)NC4=O)CO)OCC1OC(C(C1O)O)N2C=CC(=O)NC2=O.[N+H4]

InChI

1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3

Klucz InChI

VPXDHZMLJOJKOX-UHFFFAOYSA-N

Opis ogólny

Uricase deficient mice develop uric acid nephropathy and diabetes insipidus which can be mitigated with recombinant uricase treatment. [1]
Uricase is a homo-tetrameric protein with its active sites at the head-to-tail monomer-dimer interface.

Zastosowanie

Uricase from Candida sp. has been used:
  • to eliminate urate in plasma samples and evaluate the contribution of urate to the total plasma antioxidant capacity
  • to intraperitoneally inject the mice to study the role of uric acid in fibrin extracellular traps (ET) formation
  • for elimination of uric acid in ferric reducing/antioxidative power (FRAP) assay to measure the total antioxidative capacity of human plasma sample; in uricase-based biosensors

Działania biochem./fizjol.

Uricase participates in purine catabolism. It catalyzes the conversion of highly insoluble uric acid into 5-hydroxyisourate. Accumulation of uric acid causes liver/kidney damage or chronically causes gout. In mice, a mutation in the gene encoding uricase causes a sudden increase in uric acid. Mice, deficient in this gene, exhibit hyperuricemia, hyperuricosuria, and uric acid crystalline obstructive nephropathy.

Definicja jednostki

One unit will oxidize 1.0 μmole of uric acid to allantoin per minute at pH 8.5 at 25 °C.

Postać fizyczna

Powder; contains citrate and sucrose
Ta strona może zawierać tekst przetłumaczony maszynowo.

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
0000327059
0000327059
0000380939
0000349793
0000380939

Nie widzisz odpowiedniej wersji?

Jeśli potrzebujesz konkretnej wersji, możesz wyszukać konkretny certyfikat według numeru partii lub serii.

Wyszukaj dokument COA

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Klienci oglądali również te produkty

Slide 1 of 2

1 of 2

Oksydaza askorbinianowa, Cucurbita sp. Native ascorbate oxidase from Cucurbita species. Catalyzes the oxidation of ascorbioxidation of ascorbic acid to dehydroascorbic acid. Suitable for eliminating interference of ascorbic acid in clinical assays and for determination of ascorbic acid.

Sigma-Aldrich

189724

Oksydaza askorbinianowa, Cucurbita sp.

Ficin from fig tree latex saline suspension, ≥1.0 units/mg protein (biuret)

Sigma-Aldrich

F4125

Ficin from fig tree latex

Rocco Cancelliere et al.
Biosensors, 12(1) (2022-01-21)
The use of carbon nanomaterials (CNMs) in sensors and biosensor realization is one of the hottest topics today in analytical chemistry. In this work, a comparative in-depth study, exploiting different nanomaterial (MWNT-CO2H, -NH2, -OH and GNP) modified screen-printed electrodes (SPEs)
Darko Duplancic et al.
Molecules (Basel, Switzerland), 16(8), 7058-7068 (2011-08-19)
Determination of the relative contribution of uric acid level increases to the total measured antioxidative activity could be very useful for testing antioxidative products and their effect on human health. The aim of this report is to present a simple
Michael W Munks et al.
Blood, 116(24), 5191-5199 (2010-09-30)
It has been recognized for nearly 80 years that insoluble aluminum salts are good immunologic adjuvants and that they form long-lived nodules in vivo. Nodule formation has long been presumed to be central for adjuvant activity by providing an antigen
S J Kelly et al.
Journal of the American Society of Nephrology : JASN, 12(5), 1001-1009 (2001-04-24)
Uricase-deficient mice develop uric acid nephropathy, with high mortality rates before weaning. Urate excretion was quantitated and renal function was better defined in this study, to facilitate the use of these mice as a model for evaluating poly(ethylene glycol)-modified recombinant
Benoit Pugin et al.
Frontiers in microbiology, 13, 853735-853735 (2022-05-03)
Uricase catalyzes the conversion of uric acid into allantoin with concomitant reduction of molecular oxygen to hydrogen peroxide. In humans, uricase is not functional, thereby predisposing individuals to hyperuricemia, a metabolic disturbance associated with gout, chronic kidney disorders, and cardiovascular
Wyświetl wszystkie powiązane dokumenty
1 Review This action will navigate to reviews.

Questions

1–2 of 2 Questions  

  1. How can a solution in unit/mL be prepared using product number U0880, with a pack size of 250 units and SKU U0880-250UN, considering that the uricase activity is greater than 2 units/mg solid? What is the procedure for preparing a 10 unit/mL solution from this?

    1 answer

    1. You can use the calculator provided at https://www.physiologyweb.com/calculators/units_per_volume_solution_concentration_calculator.html

      Based on a specific activity of 5 units/mg, the 250 unit pack size is equivalent to 50 mg in mass. To achieve a 10 units/mL solution from this, the 50 mg would need to be dissolved in 25 mL of solvent (e.g. PBS).

      Helpful?

  2. What is the amino acid sequence of this enzyme?

    1 answer

    1. The sequence is proprietary.

      Helpful?

Reviews

Active Filters

  1. 1 Ratings-Only Review

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej