T9549
Thrombin from bovine plasma
powder, suitable for cell culture, ≥1,500 NIH units/mg protein (E1%/280 = 19.5)
Synonim(y):
Factor IIa
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About This Item
Polecane produkty
Postać
powder
aktywność właściwa
≥1,500 NIH units/mg protein (E1%/280 = 19.5)
masa cząsteczkowa
heavy chain ~31 kDa
light chain ~6 kDa
metody
cell culture | mammalian: suitable
numer dostępu UniProt
Warunki transportu
dry ice
temp. przechowywania
−20°C
informacje o genach
cow ... F2(280685)
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Opis ogólny
Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.
Zastosowanie
Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess an expression and purification system for the biosynthesis of adenosine receptor peptides for biophysical and structural characterization.
Działania biochem./fizjol.
Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.
Definicja jednostki
Activity is expressed in NIH units, and is measured by direct comparison to an NIH thrombin reference standard.
Rekonstytucja
When reconstituted with 1 mL water, vial contains stated activity in 0.15 M sodium chloride and 0.05 M sodium citrate, pH 6.5.
Komentarz do analizy
Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.
The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.
Inne uwagi
View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.
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inhibitor
Numer produktu
Opis
Cennik
Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organy docelowe
Respiratory system
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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