T9069

Topoisomerase I human

buffered aqueous glycerol solution, ≥2 units/μL

• Synonim(y): DNA Topoisomerase I
• UNSPSC Code: 12352204
• NACRES: NA.54
• Numer WE: 5.99.1.2 (BRENDA, IUBMB)
• MDL number: MFCD03458765
• Recombinant: expressed in baculovirus
• Concentration: ≥2 units/μL

Właściwości

• recombinant: expressed in baculovirus
• Quality Segment: 200
• form: buffered aqueous glycerol solution
• mol wt: 100 kDa
• concentration: ≥2 units/μL
• UniProt accession no.: P11387
• storage temp.: −70°C
• Gene Information: human ... TOP1(7150)

Opis

General description

Topoisomerase I (TOP1) is mapped to human chromosome 20q12. TOP1 is a 91 kDa type IB enzyme and consists of N-terminal highly charged domain, a core, linker domain and a C-terminal catalytic domain with Tyr-723 active site residue.

Application

Topoisomerase I has been used in a study to assess implications for the regulation of HIV-1 replication. Topoisomerase I has also been used in a study to investigate the tumor suppressor protein kinase Chk2 is a mediator of anoikis of intestinal epithelial cells. [1]

Topoisomerase I human has been used for Topoisomerase I assay for screening inhibitors.

Biochem/physiol Actions

Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right- and left-handed superhelices.

Topoisomerase I human (TOP1) interacts with DNA by wrapping and takes a bi-lobed conformation. TOPI is essential for normal development and transcriptional regulation. Variants in the TOP1 gene is observed in patients with colorectal cancer.

Physical form

Solution containing 20 mM sodium phosphate, pH 7.4, 300 mM NaCl, 50 μg/mL BSA, 50% glycerol, and between 25-100 mM imidazole (concentration will be lot dependent).

Other Notes

One unit will relax 0.25 μg of supercoiled plasmid DNA in 30 minutes at pH 7.9 at 37 °C.

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Informacja o środkach bezpieczeństwa

• pictograms: GHS08
• signalword: Danger
• hcodes: H360D
• pcodes: P201 - P308 + P313
• Hazard Classifications: Repr. 1B
• Klasa składowania: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable