In general, 'short term storage' refers to the material being stable for a period of days to weeks. In order to assure optimum performance, the end user may want to discontinue use of solutions stored refrigerated for 1 week or more as the loss of activity over time has not been investigated.
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T9003
Trypsin inhibitor
lyophilized powder
Synonim(y):
SBTI
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| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 25 mg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 289,00 zł | |
| 100 mg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 912,00 zł | |
| 250 mg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1450,00 zł | |
| 500 mg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 2840,00 zł | |
| 1 g | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 4660,00 zł | |
| 5 g | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 17 780,00 zł |
Informacje o tej pozycji
289,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócNazwa produktu
Trypsin inhibitor from Glycine max (soybean), lyophilized powder
biological source
Glycine max (soybean)
Quality Segment
form
lyophilized powder
mol wt
20.1 kDa
solubility
H2O: >10 mg/mL, phosphate buffer: >10 mg/mL (hazy above 10 mg/ml), soluble
application(s)
diagnostic assay manufacturing
storage temp.
2-8°C
Application
Trypsin has been used in a study to assess the potential application in animal cell culture of an alkaline protease from a non-toxigenic mangrove isolate of Vibrio sp. V26. Trypsin has also been used in a study to improve the detection of fungi in eosinophilic mucin. [1]
Biochem/physiol Actions
This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.
Preparation Note
The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet.
Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.
Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.
Analysis Note
One mg of trypsin inhibitor will inhibit 1.0-3.0 mg trypsin of activity ~10,000 BAEE units per mg protein.
Other Notes
One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.
One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.
The soybean trypsin inhibitor is a monomeric protein containing 181 amino acid residues in a single polypeptide chain crosslinked by two disulfide bridges.
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Ta pozycja | |||
|---|---|---|---|
| biological source Glycine max (soybean) | biological source Glycine max (soybean) | biological source Glycine max (soybean) | biological source Glycine max (soybean) |
| Quality Level 300 | Quality Level 300 | Quality Level 400 | Quality Level 100 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| solubility H2O: >10 mg/mL, soluble, phosphate buffer: >10 mg/mL (hazy above 10 mg/ml) | solubility balanced salt solution: 1 mg/mL, concentrate: >10 mg/mL, hazy, amber-yellow, phosphate buffer: 10 mg/mL, water: 10 mg/mL, serum-free medium: soluble | solubility 0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL, clear, colorless to very faintly yellow | solubility 0.1 M phosphate pH 7.6: 5 mg/mL, clear, colorless to almost colorless |
| mol wt 20.1 kDa | mol wt 20,100 Da | mol wt 20 kDa | mol wt Mr ~20000 |
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signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Klasa składowania
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protokoły
Do pomiaru aktywności inhibitora trypsyny stosuje się spektrofotometryczny test oznaczania szybkości przy 253 nm. Jedna jednostka enzymu spowoduje zmianę absorbancji przy użyciu BAEE jako substratu.
This technical article described the Enzymatic Assay of Trypsin Inhibitor.
Powiązane treści
QC Methods
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| T9003-5G | 04061837380204 |
| T9003-500MG | 04061837380174 |
| T9003-1G | 04061837380082 |
| T9003-100MG | 04061835513253 |
| T9003-250MG | 04061837380150 |
| T9003-25MG | 04061835513260 |
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what is the time period for "short term storage" at 4C after reconstitution of the powder?
1 answer-
Helpful?
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What is the molecular weight and amino acid sequence of trypsin inhibitor from Glycine max (soybean)?
1 answer-
Soybean trypsin inhibitor is a mixture of the three types. We have not characterized our trypsin inhibitor products in terms of how much of each of the three types may be present. All three have a molecular weight of between 20,000 and 20,200 daltons, so one might use 20,100 daltons as an average.The three types vary in the amino acids at a few positions. The detailed summary of the three types (or variants) was published in the following linked reference: J. Biochem. (Japan), 98, 435-448 (1985).Note that the authors use Tia, Tib and Tic to indicate the three variants.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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