93620

Trypsin inhibitor from Glycine max (soybean)

lyophilized powder, ~10000 U/mg

• Synonim(y): SBTI
• Numer CAS: 9035-81-8
• Numer WE: 232-906-9
• Numer MDL: MFCD00082103
• Kod UNSPSC: 12352202
• NACRES: NA.77

Właściwości

• pochodzenie biologiczne: Glycine max (soybean)
• Postać: lyophilized powder
• aktywność właściwa: ~10000 U/mg
• masa cząsteczkowa: M_r ~20000
• rozpuszczalność: 0.1 M phosphate pH 7.6: 5 mg/mL, clear, colorless to almost colorless
• Warunki transportu: wet ice
• temp. przechowywania: −20°C

Opis

Działania biochem./fizjol.

This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.

Definicja jednostki

1 U corresponds to the amount of inhibitor which reduces the trypsin activity by 1 BAEE-U. (1 BAEE-U is the amount of enzyme which increases the absorbance at 253 nm by 0.001 per minute at pH 7.6 and 25 °C; BAEE, Cat. No. 12880, as substrate).

One trypsin unit = A₂₅₃ of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.

Uwaga dotycząca przygotowania

The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet.

Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.

Inne uwagi

For the inhibition of proteolytic activity; Use in affinity chromatography for the purification of trypsin; Prepared by the method of M. Kunitz.

Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H317,H334
• Zwroty wskazujące środki ostrożności: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Klasyfikacja zagrożeń: Resp. Sens. 1 - Skin Sens. 1
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)