T8280

Tris Acetate-EDTA buffer

BioReagent, 10× concentrate, for molecular biology, DNase and RNase, none detected, powder blend, suitable for electrophoresis

• Synonim(y): TAE buffer
• Numer MDL: MFCD00236357
• Kod UNSPSC: 41105319
• Identyfikator substancji w PubChem: 329826878
• NACRES: NA.25

Właściwości

• klasa czystości: for molecular biology
• linia produktu: BioReagent
• Postać: powder blend
• zanieczyszczenia: DNase and RNase, none detected
• pH: 8.1-8.5
• przydatność: suitable for electrophoresis; suitable for gel electrophoresis (after dilution to working concentration)
• Zastosowanie: diagnostic assay manufacturing
• ciąg SMILES: CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)
• Klucz InChI: HGEVZDLYZYVYHD-UHFFFAOYSA-N

Opis

Zastosowanie

Ready for use in gel electrophoresis after dilution to working concentrations.

TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Opakowanie

Packaged in plastic bottles large enough to contain the concentrate after reconstitution.

Uwaga dotycząca przygotowania

Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).

Rekonstytucja

Produces a 10× concentrate (0.4 M Tris-acetate, 10 mM EDTA, pH 8.3) after dissolving with the indicated amount of water.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: dust mask type N95 (US), Eyeshields, Gloves