T7451

Anti-Acetylated Tubulin antibody, Mouse monoclonal

clone 6-11B-1, purified from hybridoma cell culture

• Synonim(y): Acetylated Tubulin Antibody, Acetylated Tubulin Antibody - Monoclonal Anti-Acetylated Tubulin antibody produced in mouse, Anti Acetyl Tubulin antibody, Anti Acetyl-alpha tubulin
• Numer MDL: MFCD00164512
• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 200
• białko sprzężone: unconjugated
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 6-11B-1, monoclonal
• Postać: buffered aqueous solution
• reaktywność gatunkowa: bovine, frog, invertebrates, human, hamster, mouse, protista, pig, monkey, chicken, rat, plant
• opakowanie: antibody small pack of 25 μL
• stężenie: ~1 mg/mL
• metody: dot blot: suitable; electron microscopy: suitable; immunohistochemistry: suitable; radioimmunoassay: suitable; western blot: 0.03-0.06 μg/mL using total rat brain extract
• izotyp: IgG2b
• numer dostępu UniProt: P68366
• Zastosowanie: research pathology
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• docelowa modyfikacja potranslacyjna: acetylation (Lys40)
• informacje o genach: human ... TUBA4A(7277) ; mouse ... Tuba1a(22142) ; rat ... Tuba1a(64158)

Opis

Opis ogólny

α-tubulin, also called as tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α -tubulin family containing 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000.

Monoclonal anti-acetylated tubulin antibody (mouse IgG2b isotype) is derived from the hybridoma 6-11B-1 produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin). Tubulin is a heterodimer, which consists of a-tubulin and b-tubulin; both subunits have a molecular weight of 50,000 and share considerable homology.

Specyficzność

The antibody has been used to detect acetylated α-tubulins from many organisms including protista, plants, invertebrates, and vertebrates. The antibody recognizes an epitope located on the α3 isoform of Chlamydomonas axonemal α-tubulin, within four residues of Lys⁴⁰ when this amino acid is acetylated. A sequence very similar to the one detected by the antibody in Chlamydomonas is found in the majority of α-tubulins, but the corre­sponding region is markedly divergent in some α-tubulin isoforms from chicken, Drosophila, and yeast. Occasionally, the epitope recognized by the antibody may be absent or masked, as it is in the rat kangaroo epithelial-like cell line PtK₂.

Immunogen

acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin).

Zastosowanie

Monoclonal Anti-Acetylated Tubulin antibody produced in mouse has been used in:

• quantitative dot blot
• immunofluorescence
• Western blot
• immunocytochemistry
• enzyme-linked immunosorbent assay (ELISA)
• solid-phase radioimmunoassay (RIA)
• electron microscopy

Anti-acetylated tubulin antibody has also been used in staining X-gal–stained paraffin sections to perform immunohistochemistry.

Działania biochem./fizjol.

Acetylated tubulin is associated with axoneme assembly in a variety of organisms. Acetylated tubulin might play a crucial role in the differentiation of microtubule structure and function. Post translational modification of α-tubulin i.e. the reversible addition of an acetyl group to Lys40, by specific acetylase, results in acetylated tubulin.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)