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T2663

Trizma® hydrochloride solution

Molecular Biology, 1 M, BioReagent

Synonim(y):

Tris hydrochloride solution

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Gabaryty przesyłkiSKUDostępnośćCena netto
1 L
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
306,00 zł

Informacje o tej pozycji

NACRES:
NA.25
PubChem Substance ID:
24900064
UNSPSC Code:
12161700
MDL number:
MFCD00012590

306,00 zł

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grade

Molecular Biology

Quality Level

200

sterility

0.2 μm filtered

product line

BioReagent

form

solution

concentration

1 M

impurities

DNase, RNase, NICKase and protease, none detected

pH

7.35-7.45

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

General description

Trizma® hydrochloride solution is a useful biological buffer.

Application

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Trizma® hydrochloride along with Trizma-base is used to prepare buffer solutions of pH 7.35.[1] It was used to prepare the triton extract.[2] It may be used in the analysis of protease activity, isolated from the Bacillus stearothermophilus and Bacillus subtilis.[3] It may be employed as buffer for the restriction fragment analysis of genomic DNA isolated from the enterococcal isolates and Escherichia coli.[4]

Other Notes

Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
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Trizma® hydrochloride solution Molecular Biology, 1 M, BioReagent

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Trizma® hydrochloride solution

Trizma® hydrochloride solution pH 7.0, BioPerformance Certified, 1 M, suitable for cell culture

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Trizma® hydrochloride solution

grade

Molecular Biology

grade

Molecular Biology

grade

BioPerformance Certified, Molecular Biology

grade

BioPerformance Certified, Molecular Biology

concentration

1 M

concentration

1 M

concentration

1 M

concentration

1 M

product line

BioReagent

product line

BioReagent

product line

-

product line

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

solution

form

solution

form

solution

form

liquid

pH

7.35-7.45

pH

8.0

pH

7.8

pH

7.0

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Klasa składowania

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Certyfikaty analizy (CoA)

Lot/Batch Number
SLCR1843
0000423330
0000251429
SLCR1843
0000423330

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

B E Murray et al.
Journal of clinical microbiology, 28(9), 2059-2063 (1990-09-01)
Epidemiologic evaluation of enterococci has been limited by the lack of a simple and effective method for comparing strains. In this study, we have compared chromosomal restriction endonuclease digestion patterns of 27 isolates of Enterococcus faecalis from three different locations
M Fujii et al.
Journal of bacteriology, 154(2), 831-837 (1983-05-01)
The structural gene for a thermostable protease from Bacillus stearothermophilus was cloned in plasmid pTB90. It is expressed in both B. stearothermophilus and Bacillus subtilis. B. stearothermophilus carrying the recombinant plasmid produced about 15-fold more protease (310 U/mg of cell
Reconstitution and purification of the D-glucose transporter from human erythrocytes.
M Kasahara et al.
The Journal of biological chemistry, 252(20), 7384-7390 (1977-10-25)
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Numer pozycji handlu globalnego

SKUNUMER GTIN
T2663-1L04061837342721
T2663-100ML04061838238313

Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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