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Merck

S8761

Sodium Bicarbonate

Solution (7.5%), sterile-filtered, suitable for cell culture

Synonim(y):

aqueous sodium bicarbonate

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100 ML

74,90 zł

500 ML

336,00 zł

74,90 zł


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Informacje o tej pozycji

Numer CAS:
UNSPSC Code:
12161702
PubChem Substance ID:
NACRES:
NA.75
MDL number:

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Nazwa produktu

Sodium bicarbonate solution, solution (7.5%), sterile-filtered, BioReagent, suitable for cell culture

SMILES string

[Na+].OC([O-])=O

InChI

1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1

InChI key

UIIMBOGNXHQVGW-UHFFFAOYSA-M

sterility

sterile-filtered

product line

BioReagent

form

solution (7.5%)

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

7.8-8.2

storage temp.

2-8°C

Quality Level

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Ta pozycja
S5761792519S6014
technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

product line

BioReagent

product line

BioReagent

product line

Redi-Dri

product line

-

Quality Level

400

Quality Level

300

Quality Level

100

Quality Level

300

form

solution (7.5%)

form

powder

form

powder

form

powder

pH

7.8-8.2

pH

6.8-7.4

pH

-

pH

-

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

-

General description

Sodium bicarbonate (NaHCo3) is a commonly used alkaline buffering agent(5) in laboratories. The addition of this buffer to the cell culture medium aids in maintaining the appropriate pH by using the carbon dioxide present in the incubator. In addition to its buffering properties, Sodium bicarbonate also acts as a source of sodium and aids in sodium gluconate synthesis during the fermentation process.

Application

Sodium bicarbonate solution has been used:
  • as a component of Hanks′ buffered salt solution (HBSS) and recording medium for culturing of tissue explants from rats or mice[1]
  • as a component of Dulbecco′s modified Eagle′s medium (DMEM) to culture mice tissue explants[2]
  • as a component of Minimum Essential Media (MEM) to study the cytotoxicity against human colorectal adenocarcinoma cells and mammary gland adenocarcinoma[3]
  • to prepare collagen mix droplets for 3D collagen sprouting assay[4]
  • to prepare collagen matrix for rat epidermal keratinocyte (REK) organotypic culture[5]

Preparation Note

Prepared in cell culture grade water.
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Klasa składowania

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Phenotypic Modulation of Adipose-Derived Stem Cells and Fibroblasts Treated with Povidone--Iodine and Chlorhexidine in Mono and Coculture Models
Chelmu? BA, et al.
Biomedicines, 11(7), 1855-1855 (2023)
Nicoline Dorothea Jakobsen et al.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 178, 106282-106282 (2022-08-23)
Rat epidermal keratinocyte (REK) Organotypic culture (ROC) is an epidermis model that is robust and inexpensive to develop and maintain, and it has in previous studies been shown to have permeability characteristics close to those of human skin. Here, we
Mette Flinck et al.
Cell cycle (Georgetown, Tex.), 17(9), 1056-1067 (2018-06-14)
Precise acid-base homeostasis is essential for maintaining normal cell proliferation and growth. Conversely, dysregulated acid-base homeostasis, with increased acid extrusion and marked extracellular acidification, is an enabling feature of solid tumors, yet the mechanisms through which intra- and extracellular pH
Anna E Engler et al.
Cell reports, 33(13), 108553-108553 (2020-12-31)
There is an increasing appreciation for the heterogeneity of myeloid lineages in the lung, but relatively little is known about populations specifically associated with the conducting airways. We use single-cell RNA sequencing, flow cytometry, and immunofluorescence to characterize myeloid cells
Caroline Volz et al.
Cell reports, 31(4), 107568-107568 (2020-04-30)
Anti-angiogenic treatment targeting vascular endothelial growth factor (VEGF)-VEGFR2 signaling has shown limited efficacy in lung cancer patients. Here, we demonstrate that inhibition of VEGFR2 in tumor cells, expressed in ∼20% of non-squamous non-small cell lung cancer (NSCLC) patients, leads to

Protokoły

3-D Culture Matrix™ Collagen I facilitates cellular growth and differentiation in vitro, offering versatile applications in biomedical research.

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