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P8170

Protein G−Peroxidase from Streptococcus sp.

recombinant, expressed in unspecified host, lyophilized powder

Synonim(y):

Protein G−HRP from Streptococcus sp.

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Do Państwa/SKUDostępnośćCena netto
250 μg
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712,00 zł

Informacje o tej pozycji

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
Form:
lyophilized powder
Recombinant:
expressed in unspecified host

712,00 zł


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Quality Segment

recombinant

expressed in unspecified host

conjugate

peroxidase conjugate

form

lyophilized powder

technique(s)

direct ELISA: 1:100,000 using human IgG

storage temp.

−20°C

General description

Protein G is a bacterial cell wall protein, which has high affinity for immunoglobulin G (IgG). It is isolated from group G streptococcal strains. Peroxidase from Streptococcus sp. is used to maintain the H2O2 homeostasis in cells. It aids the regulation of ABC Mn2+-permease complex (psaBCA) genes.

Application

Protein G- Peroxidase from Streptococcus sp. has been used in indirect ELISA and immunoblotting.

Biochem/physiol Actions

The product binds IgG for most mammalian species (excluding feline) and can be used in ELISA or immunohistochemical formats. Protein was determined to be at 250-350ug per 250ug vial by UV absorbance. Reactive to chicken IgG as well.

Preparation Note

Labeled with peroxidase type VI by a modification of the procedure of O′Sullivan, M.S., et al., FEBS Lett., 95, 311 (1978) which favors low molecular weight conjugates. Conjugate is purified by gel filtration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Ta pozycja
P8045P3296P8651
technique(s)

direct ELISA: 1:100,000 using human IgG

technique(s)

direct ELISA: 1:70,000 using human IgG

technique(s)

affinity chromatography: suitable

technique(s)

direct ELISA: 1:80,000 using human IgG

recombinant

expressed in unspecified host

recombinant

expressed in unspecified host

recombinant

expressed in E. coli

recombinant

-

form

lyophilized powder

form

lyophilized powder

form

aqueous ethanol suspension

form

lyophilized powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100, 200

conjugate

peroxidase conjugate

conjugate

biotin conjugate

conjugate

-

conjugate

peroxidase conjugate


Klasa składowania

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Questions

  1. After diluting this, how long does it keep in a fridge? (2-8 degrees Celsius)

    1 answer
    1. The solution should be stable for about one week when stored at 2-8°C. For long-term storage, the stock solution should be frozen in aliquots at -20°C.

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